Parainfluenza Virus IgA ELISA Kit

Name: Parainfluenza Virus IgA ELISA Kit
SKU: DEIA370
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The ELISA Parainfluenza Virus IgA test is a qualitative and quantitative immunoassay for the detection of human antibodies in serum or plasma directed against all relevant human pathogenic Parainfluenza Viruses.

Contents of Kit

1. Break apart microtiter test strips each with eight antigen coated single wells, (altogether 96), 1 frame. The coating material is inactivated. 12 pieces
2. Standard serum (ready-to-use), Human serum in protein-containing phosphate buffer; negative for anti-HIV Ab, HBs-Ag (Hepatitis B-Virus surface Antigen) and anti-HCV Ab; Preservative: <0.1% sodium azide; coloring: Amaranth O. 2 × 2 ml.
3. Negative control serum (ready-to-use), Human serum in protein-containing phosphate buffer; negative for anti-HIV Ab, HBs-Ag (Hepatitis B-Virus surface Antigen) and anti-HCV Ab; Preservative: <0.1% sodium azide; coloring: Lissamin Green V. 2 ml
4. Anti-human IgA conjugate (ready-to-use), Anti-human IgA polyclonal antibody, Conjugated to alkaline phosphatase, stabilized with protein stabilization solution; Preservative: <0.1% methylisothiazolone, <0.1% bromnitrodioxane. 13ml
5. Washing solution concentrate (sufficient for 1000ml), Sodium chloride solution with Tween 20 and 30mM Tris-HCl, pH 7.4; Preservative: <0.1% sodium azide. 33.3ml
6. Dilution buffer (ready-to-use), Protein-containing phosphate buffer with Tween 20; Preservative: <0.1% sodium azide; coloring: 0.01g/l Bromphenol blue. 2 × 50ml
7. Stopping solution (ready-to-use), <0.1N sodium hydroxide, 40mM EDTA. 15ml
8. Substrate (ready-to-use), Para-nitrophenylphosphate in solvent-free buffer; Preservative: <0.1% sodium azide. 13ml

Storage

Reagent	Storage	Stability
Microtiter strips (coated with antigen)	Unopened After opening at 2-8°C in closed aluminum bag with desiccant	See expiry date Minimum shelf-life: four weeks
Control sera / Standard sera	Unopened / after opening at 2-8°C	See expiry date
Conjugate	Unopened / after opening at 2-8°C	See expiry date
Dilution buffer	Unopened / after opening at 2-8°C	See expiry date
Washing solution	Unopened / after opening at 2-8°C Working dilution at 2-8°C Working dilution at room temperature	See expiry date 2 weeks 1 week
Substrate	Unopened / after opening at 2-8°C	See expiry date
Stopping solution	Unopened / after opening at 2-8°C	See expiry date

General Description

Parainfluenza Viruses cause mild to severe upper and lower respiratory tract infections. Parainfluenza Viruses are surpassed only by Respiratory Syncytial Virus as the cause of severe lower respiratory tract infections in children. Infections are transmitted by droplet infection or direct contact via mucous membranes of the eyes, mouth, or nose. Currently, Parainfluenza Viruses can be divided into four type of which types 1-3 are most frequently detected.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

Infectious Disease Test Kits and Positive Controls

QA & Reviews

Customer Reviews

References

Forces and flow induced by a moving intruder in a granular packing: coarse-graining and DEM simulations versus experiments

GRANULAR MATTER

Authors: Lehuen, Julien; Delenne, Jean-Yves; Duri, Agnes; Ruiz, Thierry

Abstract

The study of stress transmission and particles' mobility in powder beds constitutes a challenging issue to improve processes such as milling, agglomeration and kneading. In this paper the ability to promote particle mobilities by means of an intruder is investigated. An experimental setup was developed to characterize the particles' mobility at the voxel scale. A particle image velocimetry (PIV) algorithm was employed to determine the velocity fields in the vicinity of an intruder in ascendant vertical motion. A discrete element method model was developed to simulate the same system at the particle scale. Velocity maps were determined relying on a coarse-graining procedure and compared to PIV results. During the rise of the intruder the experimental and simulated drag forces are in good agreement. Spatio-temporal correlation between the granular mobility and the state of the force network are analyzed as a function of the height of the intruder. The drag force profile exhibits a relaxation trend, noised by fluctuations whose origin are in successive loading/rupture events. An interpretation of these fluctuations is proposed with regard to the development of preferential paths in the normal force network, and to the localization of sliding at the contacts scale. Finally, we show that global strains are a consequence of the ejection of particles directly in contact with the ends of the intruder.

Experimental investigations of the asymmetric vortex formation over a slender body of revolution at high angles of attack

INTERNATIONAL JOURNAL OF MODERN PHYSICS B

Authors: Zhu, Yiding

Abstract

This paper describes an experimental investigation of the initial growth of flow asymmetries over a slender body of revolution at high angles of attack with natural and disturbed noses. Time-resolved particle image velocimetry (PIV) is used to investigate the flow field around the body. Flow visualization clearly shows the formation of the asymmetric vortices. Instantaneous PIV shows that the amplified asymmetric disturbances lead to Kelvin-Helmholtz instability appearing first on one side, which increases the momentum exchange crossing the layer. As a result, the separation region shrinks which creates the initial vortex asymmetry.