Pam3CSK4

Content
1 mg of lyophilized Pam3CSK4 Vaccine grade adjuvant
1.5 ml endotoxin-free water
10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

Storage and Stability
Pam3CSK4 is shipped at room temperature and should be stored at 4 °C. Lyophilized product is stable for 1 year when properly stored.
Upon resuspension, prepare aliquots of Pam3CSK4 and store at -20 °C. Resuspended product is stable for 6 months when properly stored. Avoid repeated freeze thaw cycles.

Description
Pam3CSK4 is a synthetic triacylated lipopeptide (LP) that mimics the acylated amino terminus of bacterial LPs. Pam3CSK4 is a potent activator of the proinflammatory transcription factor NF-kB. Activation is mediated by the interaction between TLR2 and TLR1 which recognize LPs with three fatty acids, a structural characteristic of bacterial LPs.
Several TLR2 agonists, in particular lipopeptides, have been evaluated as vaccine adjuvants. Pam3CSK4 has been proven to be a potent adjuvant for various vaccines, including a sublingual allergy vaccine, flu vaccine and leishmaniasis vaccine. In a preclinical study, Pam3CSK4 was reported to increase antibody responses to flu antigens unlike other TLR ligands. It was shown to exert a strong local response, enhance IgG2a and IgG1 titers and upregulate proinflammatory and Th1 cytokine genes. Pam3CSK4 was also used as an adjuvant to improve the efficacy of a DNA-based vaccine against Leishmania. Pam3CSK4 increased antigen specific CD8 cells in immunized mice and induced higher levels of IFN-γ.

Preparation of Sterile Stock Solution (2 mg/ml)
Add 500 μl endotoxin-free water (provided) to the 1 mg Pam3CSK4 vial.
- Vortex until completely dissolved.
- Further dilutions can be prepared using sterile aqueous solutions for injection, such as the sterile endotoxin-free physiological water (provided).

Chemical Properties
CAS number: 112208-00-1
Molecular formula: C81H156N10O13S
Molecular weight: 1509.6
Solubility: 2 mg/ml in water

Vaccine Grade