Vaccine Grade

Pam3CSK4

  • Product Name
  • Pam3CSK4 / Synthetic triacylated lipoprotein
  • Cat. No.
  • ADJU-022CL
  • Content
  • 1 mg of lyophilized Pam3CSK4 Vaccine grade adjuvant
    1.5 ml endotoxin-free water
    10 ml sterile endotoxin-free physiological water (NaCl 0.9%)
  • Storage and Stability
  • Pam3CSK4 is shipped at room temperature and should be stored at 4 °C. Lyophilized product is stable for 1 year when properly stored.
    Upon resuspension, prepare aliquots of Pam3CSK4 and store at -20 °C. Resuspended product is stable for 6 months when properly stored. Avoid repeated freeze thaw cycles.
  • Description

  • Pam3CSK4 is a synthetic triacylated lipopeptide (LP) that mimics the acylated amino terminus of bacterial LPs. Pam3CSK4 is a potent activator of the proinflammatory transcription factor NF-kB. Activation is mediated by the interaction between TLR2 and TLR1 which recognize LPs with three fatty acids, a structural characteristic of bacterial LPs.
    Several TLR2 agonists, in particular lipopeptides, have been evaluated as vaccine adjuvants. Pam3CSK4 has been proven to be a potent adjuvant for various vaccines, including a sublingual allergy vaccine, flu vaccine and leishmaniasis vaccine. In a preclinical study, Pam3CSK4 was reported to increase antibody responses to flu antigens unlike other TLR ligands. It was shown to exert a strong local response, enhance IgG2a and IgG1 titers and upregulate proinflammatory and Th1 cytokine genes. Pam3CSK4 was also used as an adjuvant to improve the efficacy of a DNA-based vaccine against Leishmania. Pam3CSK4 increased antigen specific CD8 cells in immunized mice and induced higher levels of IFN-γ.
  • Preparation of Sterile Stock Solution (2 mg/ml)
  • Add 500 μl endotoxin-free water (provided) to the 1 mg Pam3CSK4 vial.
    - Vortex until completely dissolved.
    - Further dilutions can be prepared using sterile aqueous solutions for injection, such as the sterile endotoxin-free physiological water (provided).
  • Working Concentration
  • 2-20 μg/mouse
  • Chemical Properties

  • CAS number: 112208-00-1
    Molecular formula: C81H156N10O13S
    Molecular weight: 1509.6
    Solubility: 2 mg/ml in water

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