Anti-PPRC1 polyclonal antibody (CABT-BL2990)

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 700 - 800 of Human PPRC1.
Conjugate
Unconjugated

Applications


Application Notes
WB: 1 μg/ml
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
PPRC1; peroxisome proliferator-activated receptor gamma, coactivator-related 1; peroxisome proliferative activated receptor, gamma, coactivator related 1; peroxisome proliferator-activated receptor gamma coactivator-related protein 1; KIAA0595; MGC74642
Entrez Gene ID
UniProt ID

Citations


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References


PPRC1, but not PGC-1 alpha, levels directly correlate with expression of mitochondrial proteins in human dermal fibroblasts

GENETICS AND MOLECULAR BIOLOGY

Authors: Mori, Mateus Prates; de Souza-Pinto, Nadja Cristhina

The XPC protein, which is mutated in xeroderma pigmentosum (XP) complementation group C (XP-C), is a lesion recognition factor in NER, but it has also been shown to interact with and stimulate DNA glycosylases, to act as transcriptional co-activator and on energy metabolism adaptation. We have previously demonstrated that XP-C cells show increased mitochondrial H2O2 production with a shift between respiratory complexes I and II, leading to sensitivity to mitochondrial stress. Here we report a marked decrease in expression of the transcriptional co-activator PGC-1 alpha, a master regulator of mitochondrial biogenesis, in XP-C cells. A transcriptional role for XPC in PGC-1 alpha expression was discarded, as XPC knockdown did not downregulate PGC-1 alpha expression and XPC-corrected cells still showed lower PGC-1 alpha expression. DNA methylation alone did not explain PGC-1 alpha silencing. In four different XP-C cell lines tested, reduction of PGC-1 alpha expression was detected in three, all of them carrying the c.1643_1644deITG mutation (Delta TG) in XPC. Indeed, all cell lines carrying XPC Delta TG mutation, whether homozygous or heterozygous, presented decreased PGC-1 alpha expression. However, this alteration in gene expression was not exclusive to XPC Delta TG cell lines, for other non-related cell lines also showed altered PGC-1 alpha expression. Moreover, PGC-1 alpha expression did not correlate with expression levels of TFAM and SDHA, known PGC-1 alpha target-genes. In turn, PPRC1, another member of the PGC family of transcription co-activators controlling mitochondrial biogenesis, displayed a good correlation between its expression in 10 cell lines and TFAM and SDHA. Nonetheless, PGC-1 alpha knockdown led to a slight decrease of its target-gene protein level, TFAM, and subsequently of a mtDNA-encoded gene, MT-CO2. These results indicate that PGC-1 alpha and PPRC1 cooperate as regulators of mitochondrial biogenesis and maintenance in fibroblasts.

Peri-implantation lethality in mice lacking the PGC-1-related coactivator protein

DEVELOPMENTAL DYNAMICS

Authors: He, Xin; Sun, Chen; Wang, Feng; Shan, Aijing; Guo, Ting; Gu, Weiqiong; Cui, Bin; Ning, Guang

Background: Members of the PPAR? coactivator-1 (PGC-1) family are central transcriptional coactivators that regulate cell metabolic processes ranging from mitochondrial biogenesis to oxidative respiration. PGC-1-related coactivator (PPRC1 or PRC), initially identified as a member of the PGC-1 family, is believed to regulate mitochondria biogenesis, respiration pathways, and cell proliferation. However, its physiological role is not clearly understood. Here, we investigate the biological functions of PPRC1 in vivo using PPRC1 deficient mice generated by gene targeting. Results: Homozygous deficient PPRC1 mice failed to form egg cylinders and died after implantation but before embryonic day 6.5, whereas mice heterozygous for PPRC1 were viable, fertile and indistinguishable from their wild-type littermates. Furthermore, PPRC1 mRNA was expressed at the embryonic stage before implantation and was rapidly up-regulated during the first day of embryoid body formation. The PPRC1 mRNA was then subsequently down-regulated, although its precise function at this stage of development was unclear. Conclusions: This is the first study to suggest a nonredundant role for PPRC1 in mouse early embryonic development. Developmental Dynamics 241:975983, 2012. (c) 2012 Wiley Periodicals, Inc.

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