Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The PIK3C2B knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Alternative Names
PIK3C2B; phosphoinositide-3-kinase, class 2, beta polypeptide; phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta; C2 PI3K; PI3K C2beta; PI3K-C2beta; PI3K-C2-beta; PTDINS-3-kinase C2 beta; ptdIns-3-kinase C2 subunit beta; phosphoinositide 3-kinase-C2-beta; phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide; C2-PI3K; DKFZp686G16234;
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Concentration
The protein concentration was determined with BCA assay.
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
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Arcaro, A; Zvelebil, MJ; et al. Class II phosphoinositide 3-kinases are downstream targets of activated polypeptide growth factor receptors. MOLECULAR AND CELLULAR BIOLOGY 20:3817-3830(2000).
Muffak-Granero, K; Olmedo, C; et al. Gene Network Profiling Before and After Transplantation in Alcoholic Cirrhosis Liver Transplant Recipients. TRANSPLANTATION PROCEEDINGS 44:1493-1495(2012).
COA
Certificate of Analysis
