Mouse Anti-Porcine Epidemic Diarrhea Virus Monoclonal Antibody, clone 3G8

In 2014, porcine epidemic diarrhea (PED) outbreaks characterized by mild clinical disease and low pre-weaning mortality increased in frequency in Thailand. An outbreak investigation, based on the detection of the classical variant and the allowance of the herd owners, was conducted in 5 swine herds and 25 intestinal samples were submitted from herds with outbreaks of diarrhea for sequencing analysis. PEDV isolation and complete nucleotide sequences of the spike (S) glycoprotein were determined. The PEDV isolate EAS1 was identified. Phylogenetic analysis based on the full-length spike gene demonstrated that EAS1 belonged to the classical variant group with high nucleotide and amino acid identity (98.4-99.0% and 96.6-97.9%) with LZC and SM98, which are the Chinese and Korean classical variant PEDVs, respectively. The results suggest the emergence of a classical variant PEDV responsible for the mild clinical disease in Thailand. The introduction could have been due to the use of illegally imported modified live vaccines from other countries.

Porcine epidemic diarrhea virus(PEDV) causes enteritis, vomiting, watery diarrhea, and high mortality in suckling pigs, threatening the swine industry. Porcine epidemic diarrhea (PED) re-emerged globally in 2013 in many important swine-producing countries in Asia and the Americas. Several studies have identified the risk factors for the spread of PEDV in acute outbreaks. However, limited information is available on the risk factors for the transmission of PEDV in endemic regions. We hypothesized that poor biosecurity, location, and some social or cultural practices are the main risk factors for PEDV transmission in the Vietnamese pig population. The aim of this study was to evaluate the potential risk factors for the transmission of PEDV in an endemic area in Vietnam. In this case-control study, questionnaires containing 51 questions were completed for 92 PEDV-positive and 95 PEDV-negative farms. A logistic regression analysis was performed to assess the risk factors associated with PEDV infection. Province and the total number of pigs were included as random effects to determine their influence on the risk of PEDV infection. Twenty-nine variables of interest that have been associated with PEDV status were analyzed in a univariate analysis (P<0.20), with backward stepwise selection. Only three of these 29 variables in four models remained significant PEDV risk factors in the final model: farrow-to-wean production type, distance from the farm to the slaughterhouse (<1,000 m), and the presence of chickens on site (P<0.05). This is the first study to identify the main risk factors for PEDV infection in an endemic area. Our findings suggest that hygiene measures should be strictly implemented on farms for the effective control and prevention of PEDV infection.