Anti-PDP1 polyclonal antibody

The goal of this study is to characterize the BrCSR (B. rapa Cold Stress Resistance) gene and to analyze the expression pattern of genes related to cold response under low temperatures in Chinese cabbage. A transgenic Chinese cabbage line was generated using expression vector pSL101 containing the BrCSR full length CDS. Five transgenic plants of T-1-progeny that were selected by PCR and southern hybridization showed approximately 2-fold higher expression than the wild-type control under cold stress conditions. These transgenic plants showed less susceptibility to chilling injury compared to the control. To evaluate genes that are functionally related to BrCSR and cold-responsive genes, a gene co-expression network had been preferentially constructed, and then B. rapa 135K cDNA microarray was subsequently analyzed. BrCSR was strongly associated with PDP1, NYC1, and CYP72A11, which are involved in the cold stress tolerance response. Expression levels of genes related to the biosynthesis of succinate and thiamine, which have reported to be associated with cold tolerance, significantly changed in the gene co-expression network. Taken together, these results indicated that BrCSR plays a significant role in the adaptation of plants to low temperature conditions.

Cerebral pyruvate depletion and lactate acidosis are common metabolic characteristics of patients with traumatic brain injury (TBI) and are associated with poor prognosis. Pyruvate dehydrogenase (PDH) is the rate-limiting enzyme coupling glycolysis to mitochondrial tricarboxylic acid (TCA) cycle. Brain PDH activity is regulated by its phosphorylation status and other effectors. Phosphorylation of PDH E1 alpha 1 subunit by PDH kinase inhibits PDH activity while dephosphorylation of phosphorylated PDHE1 alpha 1 by PDH phosphatase (PDP1) restores PDH activity. In situ hybridization showed that PDP1 mRNA is highly expressed in the cerebral cortex, hippocampus and thalamus of rat. Controlled cortical impact (CCI) induced a significant increase in PDP1 mRNA expression in ipsilateral cerebral cortex at 4h (P<0.05) and 24h post CCI (P<0.01) that returned to basal level 72h post CCI. PDP1 mRNA level increased transiently in ipsilateral hippocampal dentate gyrus and CA1-3 subfields 4 h post CCI (P<0.01) but decreased significantly 24 h and 72 h (P<0.01) post CCI, coinciding with a marked increase in neuronal apoptosis in ipsilateral hippocampus 24 h post CCI. PDP1 mRNA expression in thalamus and other subcortical regions decreased persistently post CCI. Contralateral CCI and craniotomy showed similar effects on PDP1 mRNA expression as ipsilateral CCI. Because GFAP mRNA expression was induced in brain regions where PDP1 expression was altered, further study should determine the potential relationship between astrocyte activation, PDP1 alteration, and pyruvate metabolism following TBI. (C) 2012 Elsevier Ireland Ltd. All rights reserved.