PD-1/PD-L1 (Biotiny) Inhibitor Screening Colorimetric Assay Kit

Intended Use

The PD-1/PD-L1 Inhibitor Screening Colorimetric Assay Kit is designed for screening inhibitors of PD-L1 ligand binding to PD-1 receptor.

Contents of Kit

1. PD-L1, Biotin-labeled: 5 μg, -80°C;
2. PD-1: 10 μg, -80°C;
3. Streptavidin-HRP: 15 μl, +4°C;
4. 3x PD-1 Assay Buffer: 50 ml, -20°C;
5. Blocking Buffer: 50 ml, +4°C;
6. Colorimetric HRP substrate: 10 ml, +4°C;
7. Transparent 96-well microplate: 1, +4°C .

Storage

One year from date of receipt when stored as directed.

General Description

Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD-1:PDL1[Biotinylated] Inhibitor Screening Colorimetric Assay Kit is designed for screening inhibitors of PD-L1 ligand binding to PD-1 receptor. The key to this kit is the high affinity of biotin-labeled PD-L1 for streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated on a 96-well plate. Next, PD-L1 is incubated with PD-1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.