Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The PAK2 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Alternative Names
PAK2; p21 protein (Cdc42/Rac)-activated kinase 2; PAK65; PAKgamma; serine/threonine-protein kinase PAK 2; p58; PAK-2; gamma-PAK; S6/H4 kinase; p21-activated kinase 2; p21 (CDKN1A)-activated kinase 2;
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Concentration
The protein concentration was determined with BCA assay.
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
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Ding, JB; Knaus, UG; et al. The renaturable 69- and 63-kDa protein kinases that undergo rapid activation in chemoattractant-stimulated guinea pig neutrophils are p21-activated kinases. JOURNAL OF BIOLOGICAL CHEMISTRY 271:24869-24873(1996).
Tsai, IC; Hsieh, YJ; et al. Anti-phosphopeptide antibody, P-STM as a novel tool for detecting mitotic phosphoproteins: Identification of lamins A and C as two major targets. JOURNAL OF CELLULAR BIOCHEMISTRY 94:967-981(2005).
COA
Certificate of Analysis
