1 mg of lyophilized ODN 2395 Vaccine grade adjuvant
10 ml sterile endotoxin-free physiological water (NaCl 0.9%)
Storage and Stability
ODN 2395 is shipped at room temperature and should be stored at -20°C for up to 1 year.
- Upon resuspension, prepare aliquots of ODN 2395 and store at -20°C.Resuspended product is stable 6 months when properly stored. Avoid repeated freeze-thaw cycles.
ODN 1585 Sequence
5’-tcgtcgttttcggcgc:gcgccg-3’ (22 mer)
Note: Bases are phosphorothioate, palindrome is underlined.
Synthetic oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs) have been extensively studied as adjuvants. These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. CpG ODNs are recognized by TLR9, which is expressed exclusively on human B cells and plasmacytoid dendritic cells (pDCs), thereby inducing Th1-dominated immune responses. Pre-clinical studies, conducted in rodents and non-human primates, and human clinical trials have demonstrated that CpG ODNs can significantly improve vaccine-specific antibody responses.
Three types of stimulatory CpG ODNs have been identified, types A, B and C, which differ in their immune-stimulatory activities. Furthermore, CpG ODNs activate TLR9 in a species-specific manner. ODN 2395 is a type C CpG ODN specific for mouse TLR9. Type C CpG ODNs contain a full phosphorothioate backbone with one or more CpG dinucleotides. Type C CpG ODNs are potent inducers of IFN-α from pDC and strong B cell activators. In vivo studies have demonstrated that type C ODNs which combine the effects of types A and B ODNs, such ODN 2395, are very potent Th1 adjuvants.
Preparation of Sterile Stock Solution (2 mg/ml)
Add 500 μl endotoxin-free physiological water to the 1 mg ODN 2395 vial to obtain a solution at 2 mg/ml.
- Mix the solution by pipetting up and down.