Vaccine Grade

ODN 1585

  • Product Name
  • ODN 1585 / CpG ODN, type A (mouse)
  • Cat.No.
  • ADJU-031CL
  • Content
  • 1 mg of lyophilized ODN 1585 Vaccine grade adjuvant
    10 ml sterile endotoxin-free physiological water (NaCl 0.9%)
  • Storage and Stability
  • ODN 1585 is shipped at room temperature and should be stored at -20°C. Lyophilized product is stable 1 year when properly stored.
    - Upon resuspension, prepare aliquots of ODN 1585 and store at -20°C. Resuspended product is stable 6 months when properly stored. Avoid repeated freeze-thaw cycles.
  • ODN 1585 Sequence
  • 5’- ggGGTCAACGTTGAgggggg -3’ (20 mer)
    Note: Bases in capital letters are phosphodiester and those in lower case are phosphorothioate.
  • Description

  • Synthetic oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs), such as ODN 1585, have been extensively studied as adjuvants. These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. CpG ODNs are recognized by TLR9, which is expressed exclusively on human B cells and plasmacytoid dendritic cells (pDCs), thereby inducing Th1-dominated immune responses. Pre-clinical studies, conducted in rodents and non-human primates, and human clinical trials have demonstrated that CpG ODNs can significantly improve vaccine-specific antibody responses. Three types of stimulatory CpG ODNs have been identified, types A, B and C, which differ in their immune-stimulatory activities. Furthermore, CpG ODNs activate TLR9 in a species-specific manner. ODN 1585 is a type A CpG ODN specific for mouse TLR9. Type A CpG ODNs are characterized by a phosphodiester central CpG-containing palindromic motif and a phosphorothioate 3’ poly-G string. They induce high IFN-a production from plasmacytoid dendritic cells (pDC) but are weak stimulators of TLR9-dependent NF-kB signaling.
    ODN 1585 has been tested as an adjuvant in animal models. Research has demonstrated that ODN 1585 provides protective immunity against HPV16-associated-tumors in mice when combined with a peptide vaccine.
  • Preparation of Sterile Stock Solution (2 mg/ml)
  • Add 500 μl endotoxin-free physiological water to the 1 mg ODN 1585 vial to obtain a solution at 2 mg/ml.
    - Mix the solution by pipetting up and down.
  • Working Concentration
  • 20-50 μg/mouse

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