1 mg of lyophilized ODN 1585 Vaccine grade adjuvant
10 ml sterile endotoxin-free physiological water (NaCl 0.9%)
Storage and Stability
ODN 1585 is shipped at room temperature and should be stored at -20°C. Lyophilized product is stable 1 year when properly stored.
- Upon resuspension, prepare aliquots of ODN 1585 and store at -20°C. Resuspended product is stable 6 months when properly stored. Avoid repeated freeze-thaw cycles.
ODN 1585 Sequence
5’- ggGGTCAACGTTGAgggggg -3’ (20 mer)
Note: Bases in capital letters are phosphodiester and those in lower case are phosphorothioate.
Synthetic oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs), such as ODN 1585, have been extensively studied as adjuvants. These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. CpG ODNs are recognized by TLR9, which is expressed exclusively on human B cells and plasmacytoid dendritic cells (pDCs), thereby inducing Th1-dominated immune responses. Pre-clinical studies, conducted in rodents and non-human primates, and human clinical trials have demonstrated that CpG ODNs can significantly improve vaccine-specific antibody responses. Three types of stimulatory CpG ODNs have been identified, types A, B and C, which differ in their immune-stimulatory activities. Furthermore, CpG ODNs activate TLR9 in a species-specific manner. ODN 1585 is a type A CpG ODN specific for mouse TLR9. Type A CpG ODNs are characterized by a phosphodiester central CpG-containing palindromic motif and a phosphorothioate 3’ poly-G string. They induce high IFN-a production from plasmacytoid dendritic cells (pDC) but are weak stimulators of TLR9-dependent NF-kB signaling.
ODN 1585 has been tested as an adjuvant in animal models. Research has demonstrated that ODN 1585 provides protective immunity against HPV16-associated-tumors in mice when combined with a peptide vaccine.
Preparation of Sterile Stock Solution (2 mg/ml)
Add 500 μl endotoxin-free physiological water to the 1 mg ODN 1585 vial to obtain a solution at 2 mg/ml.
- Mix the solution by pipetting up and down.