Sample
Cell culture supernatant,vaccine and milk
Intended Use
This kit can be used in quantitative and qualitative analysis of neomycin residue in cell culture supernatant,vaccine and milk.
Contents of Kit
1. Microtiter plate with 96 wells coated with antigen
2. Neomycin standard solutions(6×1ml/bottle) 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb, 40.5ppb
3. Spiking standard solution: 1ml, 1ppm
4. Antibody solution (7ml)
5. Enzyme conjugate (7ml)
6. Substrate solution (2×6ml)
7. Stop solution (7ml)
8. 20×concentrated wash solution (50ml)
9. 1×concentrated extraction solution (50ml)
Storage
Storage condition: 2-8°C.
Storage period: 12months.
Performance Characteristics
Accuracy: 70-120%
Precision
Variation coefficient of the ELISA kit is less than 10%.
Detection Limit
Cell culture supernatant/Vaccine, 0.5ppb
Milk, 7.5ppb
General Description
Neomycin is an aminoglycoside antibiotic, which is broadly applied in animal disease treatment and vaccine production. For it has neurotoxicity and kidney toxicity, its residue in animal derived food is harmful to human; it is strictly controlled in use in EU, US and China. At present, ELISA is the common approach in supervision and control of aminoglycoside drug.
This kit is a new product for drug residual detection based on ELISA technology, which only costs 45min in each operation and can considerably minimize operation errors and work intensity.
Citations
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Neomycin is a broad-spectrum antibiotic belonging to the class of aminoglycosides. It is widely used in both clinical and veterinary medicine for the treatment and prevention of bacterial infections. Neomycin is effective against a range of Gram-positive and Gram-negative bacteria, making it a valuable tool in combating various types of bacterial pathogens. Neomycin works by inhibiting bacterial protein synthesis, specifically targeting the 30S ribosomal subunit. This interference with protein synthesis leads to the disruption of bacterial cell growth and ultimately results in cell death.
Figure 1. General mechanism of action representing bactericidal action of neomycin.
(Source: Abbot, V. et al., 2022)
The antibiotic is available in various formulations, including oral, topical, and ophthalmic preparations. It is commonly used to treat bacterial infections of the skin, eyes, ears, urinary tract, and gastrointestinal tract. Neomycin is also utilized as a prophylactic agent to prevent infections in surgical procedures and as a component of combination therapies for more severe bacterial infections. In addition to its medical applications, neomycin has been used in the food industry as a growth promoter in animal feed and as a preservative in certain food products.
Neomycin is generally well-tolerated, but like other aminoglycosides, it can have side effects, particularly when administered systemically. These side effects may include kidney toxicity, hearing loss, and damage to the vestibular system. It is important to monitor neomycin levels in clinical settings to ensure optimal dosing, minimize toxicity, and maximize therapeutic efficacy. At present, ELISA is commonly preferred as a simple and reliable method in the supervision and control of aminoglycoside drugs. This kit is a competitive-based ELISA that can be used for the determination of this antibiotic in cell culture supernatant, vaccine, and milk.
Alternative Names
Neomycin
Neomycin ELISA
Neomycin (Neo) ELISA
NEO ELISA
Neomycin (NMC) ELISA
Neomycin (Neo) ELISA Kit
NEO ELISA Kit
Neomycin (NMC) ELISA kit
References
1. Abbot V, et al. Development, Characterization and In Vitro Antimicrobial Evaluation of Novel Flavonoids Entrapped Micellar Topical Formulations of Neomycin Sulfate. Journal of Pharmaceutical Sciences. 2022,11(12): 3287-3296.
Q & A
Q: Can this ELISA kit used for quantitative analysis of Neomycin in cheese?
A: Our Neomycin ELISA Kit can work for egg, chicken, vaccine and milk samples. We haven't tested cheese yet.
Customer Reviews
Reconstruction of the Neopulmonary Root After Coronary Button Harvest for Arterial Switch Operation Using 2-ply Extracellular Matrix (Tyke): A Post-Implant Histology
FRONTIERS IN CARDIOVASCULAR MEDICINE
Authors: Bibevski, Steve; Ruzmetov, Mark; Ladich, Elena; Mendoza, Laura E.; Scholl, Frank G.
Abstract
In children with Transposition of the Great Arteries (TGA), the pulmonary artery, and aorta are connected to the heart abnormally resulting in blue blood (deoxygenated) recirculating to the body and red blood (oxygenated) recirculating to the lungs. The arterial switch operation (ASO) is the standard of care for transposition of the great arteries (TGA), and given the low risk of early mortality and satisfactory long-term outcomes, focus is now on managing longer term complications such as neo-aortic root dilatation, and pulmonary artery stenosis. Since May 2016, we have used 2-ply extracellular matrix (ECM; Tyke) for reconstruction of the coronary button defects using a pantaloon patch. We present histology of implanted 2-ply ECM (Tyke) from a patient who went back to surgery for development of subaortic stenosis similar to 12 months after ASO.
SABR-BRIDGE:StereotacticABlativeRadiotherapyBeforeResection to AvoIdDelay for Early-Stage LunGCancer or OligomEts During the COVID-19 Pandemic
FRONTIERS IN ONCOLOGY
Authors: Kidane, Biniam; Spicer, Jonathan; Kim, Julian O.; Fiset, Pierre-Olivier; Abdulkarim, Bassam; Malthaner, Richard; Palma, David
Abstract
Surgical resection is the standard-of-care approach for early-stage non-small cell lung cancer (NSCLC). Surgery is also considered an acceptable standard infit patients with oligometastatic lesions in the lungs. The COVID-19 pandemic has led to worldwide issues with access to operating room time, with patients and physicians facing uncertainty as to when surgical resection will be available, with likely delays of months. Further compounding this are concerns about increased risks of respiratory complications with lung cancer surgery during active phases of the pandemic. In this setting, many thoracic oncology teams are embracing a paradigm where stereotactic ablative radiotherapy (SABR) is used as a bridge, to provide radical-intent treatment based on a combination of immediate SABR followed by planned surgery in 3-6 months. This pragmatic approach to treatment has been named SABR-BRIDGE (Stereotactic ABlative Radiotherapy Before Resection to avoId Delay for early-stage lunG cancer or oligomEts). This term has also been applied to the pragmatic study of the outcomes of this approach. In this paper, we discuss the standards of care in treatment of early-stage (NSCLC) and pulmonary oligometastases, the impetus for the SABR-BRIDGE approach, and the controversies surrounding assessment of pathological response to neo-adjuvant radiation therapy.
Neomycin therapy: its use in virus pneumonia, tuberculosis and diseases caused by gram-negative bacteria
Journal of the American Medical Association
Authors: Kadison E R, Volini I F, Hoffman S J, et al.
Abstract
Neomycin, an antibiotic isolated by Waksman and his associates1 from Actinomyces fradii, was tested by its discoverers against selected organisms and by our group2 against 370 strains of bacteria, viruses and protozoa in vitro and in experimental animals. The average inhibitory concentration of neomycin expressed in units per cubic centimeter of test medium in vitro was 0.1 for Listeria, 0.9 for Salmonella typhosa, 0.16 for paratyphoid bacilli, 1.3 for other Salmonella, 1.0 for Shigella, 0.44 for Escherichia, 1.9 for Aerobacter, 1.8 for Klebsiella, 1.05 for Proteus, 3.1 for Pseudomonas, 0.8 for Brucella, 0.12 for Pasteurella, 0.4 for cholera vibrios, 0.1 for Mycobacterium tuberculosis, 0.15 for Treponemataceae and 4.7 for Endameba histolytica; the range of activity varied from doses equivalent to approximately one tenth of the average to about twenty times higher concentrations. Neomycin was active against both streptomycin sensitive and streptomycin resistant tubercle and typhoid bacilli.
Neomycin Toxicity Revisited
Archives of Surgery
Authors: Masur H, Whelton P K, Whelton A.
Abstract
Nephrotoxicity and ototoxicity represent the most hazardous side effects of the clinical use of neomycin sulfate. Despite therapeutic restriction of the latter compound to topical, irrigant, and bowel sterilization use, serious toxicity is still encountered. A 69-year-old patient was recently treated by us for acute renal failure and total deafness induced as a result of intermittent seven-day lavage of a surgical cavity with neomycin. Peritoneal dialysis reduced the serum concentration of the antibiotic and promoted complete recovery of renal function. The patient, however, remained deaf. This case serves as a reminder that neomycin can be absorbed systemically following its use as an irrigant solution. In such cases, it may produce an unsuspected form of "high output" renal failure and concomitant hearing loss. The renal failure is usually reversible, but the hearing loss is frequently permanent.
COA
Certificate of Analysis![Neomycin [BSA] (DAGA-041B)](http://img2.creative-diagnostics.com/productimages-1/Neomycin [BSA].jpg)
