Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The NFKB1 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Alternative Names
NFKB1; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B; nuclear factor NF-kappa-B p105 subunit; NF-kappabeta; DNA binding factor KBF1; DNA-binding factor KBF1; nuclear factor NF-kappa-B p50 subunit; nuclear factor kappa-B DNA binding subunit;
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Concentration
The protein concentration was determined with BCA assay.
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
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Balla, B; Kosa, JP; et al. Transcriptional profiling of immune system-related genes in postmenopausal osteoporotic versus non-osteoporotic human bone tissue. CLINICAL IMMUNOLOGY 131:354-359(2009).
Zhou, DH; Yu, T; et al. Effects of NF-kappa B1 (p50) targeted gene disruption on ionizing radiation-induced NF-kappa-B activation and TNF alpha, IL-1 alpha, IL-1 beta and IL-6 mRNA expression in vivo. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 77:763-772(2001).
COA
Certificate of Analysis
