Species Reactivity
Human, Mouse
Intended Use
The NFAT3 (Phospho-Ser676) Colorimetric Cell-Based ELISA Kit allows for the qualitative detection of NFAT3 (Phospho-Ser676) and the effects that certain stimulation conditions have on NFAT3 (Phospho-Ser676) expression in different cell lines.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 2 Plates.
2. 10x TBS: 24 mL (10x), Clear.
3. Quenching Buffer, 24 mL (1x), Clear.
4. Blocking Buffer, 50 mL (1x), Clear.
5. 10x Wash Buffer, 50 mL (10x), Clear.
6. 100x Anti-NFAT3 (Phospho-Ser676) Antibody (Rabbit Polyclonal) 60 μL (100x) Red.
7. 100x Anti-NFAT3 Antibody (Rabbit Polyclonal) 60 μL (100x) Purple.
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 μL (100x) Green.
9. HRP-Conjugated Anti-Rabbit IgG Antibody, 12 mL (1x), Glass.
10. HRP-Conjugated Anti-Mouse IgG Antibody, 12 mL (1x), Glass.
11. Primary Antibody Diluent, 12 mL (1x), Clear.
12. Ready-to-Use Substrate, 12 mL (1x), Brown.
13. Stop Solution, 12 mL (1x), Clear.
14. Crystal Violet Solution, 12 mL (1x), Glass.
15. SDS Solution, 24 mL (1x), Clear.
16. Adhesive Plate Seals, 4 Seals.
Citations
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Joshi, N; Johnson, LL; et al. Gene expression differences in normal esophageal mucosa associated with regression and progression of mild and moderate squamous dysplasia in a high-risk Chinese population. CANCER RESEARCH 66:6851-6860(2006).
Yang, TTC; Yu, RYL; et al. Integration of protein kinases mTOR and extracellular signal-regulated kinase 5 in regulating nucleocytoplasmic localization of NFATc4. MOLECULAR AND CELLULAR BIOLOGY 28:3489-3501(2008).
COA
Certificate of Analysis
