5 mg N-glycolyl-MDP Vaccine grade adjuvant.
10 ml sterile endotoxin-free physiological water (NaCl 0.9%)
Storage and Stability
N-glycolyl-MDP is provided as a sterile white lyophilized powder and shipped at room temperature. Store at -20°C.
Lyophilized product is stable for 1 year at -20°C.
Upon resuspension, N-glycolyl-MDP should be aliquoted and stored at -20°C. Resuspended product is stable for 6 months at -20°C when properly stored. Avoid repeated freeze-thaw cycles.
MDP (muramyl dipeptide), is the minimal bioactive peptidoglycan motif common to all bacteria and the essential structure required for adjuvant activity in vaccines. MDP has been shown to be recognized by NOD2, but not TLR2, nor TLR2/1 or TLR2/6 associations. The cell wall of mycobacteria is known to be extremely immunogenic. This potent activity is attributed to their MDP which is N-glycolylated in contrast to the MDP of most bacteria which is N-acetylated. N-glycolyl-MDP has been reported to display a stronger NOD2-mediated activity than N-acetyl-MDP and thus to be a more potent vaccine adjuvant than N-acetyl-MDP.
Furthermore MDP leads to the activation of the NLRP3 inflammasome.
Preparation of Sterile Stock Solution (5 mg/ml)
Add 1 ml of endotoxin-free physiological water provided to the 5 mg N-glycolyl-MDP vial to obtain a solution at 5 mg/ml.
- Vortex to homogenize.
- Prepare aliquots of stock solution and store at -20°C.