Mug (Rat) ELISA Kit is for the quantitative measurement of Mug in plasma and serum.
Contents of Kit
1. Anti-rat murinoglobulin antibody coated plate: 96 wells (12 detachable strips of 8) 2. Reference standard: 2 μg/ml (lyophilized) 3. 10× Diluent: 25 ml 4. Enzyme Conjugate Reagent: 11 ml 5. 20× Wash Solution: 50 ml 6. TMB Reagent (One-step): 11 ml 7. Stop Solution (1N HCl): 11 ml
Storage
The kit should be stored at 2-8°C and the microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air. Test kits will remain stable for six months from the date of purchase provided that the components are stored as described above.
General Description
Murinoglobulin is a negative acute phase reactant, the levels of which decrease in rat serum or plasma as a result of injury, infection or disease. In a rat adjuvant induced arthritis model murinoglobulin levels decrease by approximately eight fold 1 . Murinoglobulin therefore provides an excellent marker of inflammation/disease in rats.
Standard Curve
A typical standard curve with optical density readings at 450nm on the Y axis against murinoglobulin concentrations on the X axis is shown below. This curve is for the purpose of illustration only and should not be used to calculate unknowns. Each user should obtain his or her data and standard curve in each experiment.
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