Intended Use
The mouse immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of mouse IgG in cell culture supernatant and ascitic fluid.
The kit includes ready-to-use reagents necessary to analyze up to 90 samples in 30 min.
Buffer solutions are color coded in order to simplify pipetting steps.
Storage
All kit components are stable for 12 months when stored at 2-8°C. Do not freeze. After opening, reagents must be handled with care to avoid contamination and should be used within 2 months.
Sensitivity
The detection range is from 20 ng/mL to 1900 ng/mL.
The detection threshold is 6 ng/mL.
General Description
An antibody (Ab), also known as an immunoglobulin (Ig), is a large Y-shape protein produced by plasma cells that is used by the immune system to identify and neutralize foreign objects such as bacteria and viruses. The antibody recognizes a unique part of the foreign target, called an antigen. Each tip of the "Y" of an antibody contains a paratope (a structure analogous to a lock) that is specific for one particular epitope (similarly analogous to a key) on an antigen, allowing these two structures to bind together with precision. Using this binding mechanism, an antibody can tag a microbe or an infected cell for attack by other parts of the immune system, or can neutralize its target directly (for example, by blocking a part of a microbe that is essential for its invasion and survival). The production of antibodies is the main function of the humoral immune system.
Datasheet