Serum, plasma, tissue homogenates and other biological fluids
Species Reactivity
Mouse
Detection Method
sELISA
Intended Use
For quantitative detection of IL-1β in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
Storage
2-8°C for 6 months
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Detection Range
12.5-800pg/ml
Sensitivity
7.5pg/ml
Standard Curve
Results of a typical standard operation of a IL-1β ELISA Kit are listed below. This standard curve was generated at our lab for demonstration purpose only. Users shall obtain standard curve as per experiment by themselves. (N/A=not applicable)
Citations
Publication ()
Have you cited DEIA194 in a publication? Let us know and earn a reward for your research.
miR-130b participates in wear particle-induced inflammation and osteolysis via FOXF2/NF-κB pathway
Applications: ELISA Reactive species: Mouse
"Abstract: Objective:To reveal other miR-130b-mediated signaling pathway in the involvement of wear particle-induced inflammation and osteolysis. Materials and methods:Particle-induced osteolysis (PIO) mice model was established. Secretion levels of TNF-α, IL-1β, IL-6, and IL-10 were measured by ELISA. miR-130b and forkhead box F2 (FOXF2) mRNA were detected by qRT-PCR. Protein levels of FOXF2, phosphorylation-p65 (p-p65), and p-IκB were observed by Western blot. Luciferase reporter assay was performed to confirm the regulation of miR-130b on FOXF2." Article snippet: The protein expressions of TNF-a, IL-1b, IL-6, and IL-10 in PIO periosteum tissues and Raw264.7 cells were quantified by Mouse TNF-a ELISA KIT (Creative Diagnostics, New York, CA, USA), Mouse IL-1b ELISA KIT (Creative Diagnostics), Mouse IL-6 ELISA KIT (*), and Mouse IL-10 ELISA KIT (*) according to the manufacturer’s instructions.
Figure 1. miR-130b, FOXF2, and p-p65 expressions in Raw264.7 cell polarization. Raw264.7 cells were divided into two groups: control group and Co-Cr-Mo group. Secretion level of IL-1β was measured by ELISA.
Figure 2. miR-130b participated in osteolysis M1 macrophages polarization. M1 macrophages were divided into four groups: control group, Co-Cr-Mo group, CoCr-Mo þ NC group, and Co-Cr-Mo þ miR-130b inhibitor group. Secretion level of IL-1β was measured by ELISA.
Figure 3. miR-130b affected Co-Cr-Mo induced macrophages polarization by inhibiting FOXF2 expression to activate the NF-jB pathway. RAW264.7 cells were divided into six groups: control group, Co-Cr-Mo group, Co-Cr-Mo þ NC group, Co-Cr-Mo þ miR-130b inhibitor group, Co-Cr-Mo þ miR-130b inhibitor þ FOXF2 siRNA group, and Co-Cr-Mo þ miR-130b inhibitor þ FOXF2 siRNA þ BAY 11–7082 (10 mM) group. Secretion level of IL-1β was measured by ELISA.
My Review for Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
Creative Diagnostics products are for RESEARCH USE ONLY, please make sure your review is research based.
Required fields are marked with *
Terms and conditions:
We will select high-quality review customers and offer a $30 coupon for your next purchase.
All product reviews must be submitted in the English language.
Creative Diagnostics will not share any personal information of applicants, and all information will be treated with strict confidentiality and will not be sold or disclosed to a third party.
COA
Publication 
Loading ......
