C-Peptide (U-type) Mouse ELISA Kit (DEIA4507)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cell culture medium, citrate plasma, EDTA plasma, heparin plasma, serum
Species Reactivity
Mouse
Intended Use
This is an ELISA (Enzyme Linked ImmunoSorbent Assay) kit for measurement of mouse C-peptide with high sensitivity using Sandwich assay principle.
Contents of Kit
1. Anti-C-peptide-coated plate: 96 wells (8 x 12)/1 plate
2. Standard mouse C-peptide solution (6000pg/mL): 500 μL/1 vial
3. Buffer solution: 60 mL/1 vial
4. Biotin-conjugated anti-C-peptide: 100 μL/1 vial
5. Peroxidase-conjugated streptavidin: 100 μL/1 vial
6. Chromogenic substrate reagent (TMB): 12 mL/1 bottle l
7. Reaction stopper (1M H2SO4): 12 mL/1 bottle
8. Concentrated washing buffer (10x): 100 mL/1 bottle
Storage
2-8°C, in a dark place. Do not freeze. Valid period is 6 months afterpreparation. Expiration date is shown on the label of the container.
Performance Characteristics
Maximizing Kit Performance
1. Given the small sample volumes required (5 μL), pipetting should be done as carefully as possible. A high quality 10 μL or better precision pipette should be used for such volumes. Drops of liquid adhering to the outside of the pipette tips should be removed by wiping to ensure the highest degree of accuracy.
2. In order to prevent the microplate wells from drying out and to get the best results, samples and reagents should be dispensed quickly into the wells.
3. The wash procedure should be done thoroughly in order to minimize background readings.
4. Each calibrator and sample should be assayed in duplicate.
5. The same sequence of pipetting and other operations should be maintained in all procedures.
6. Do not mix reagents that have different lot numbers.
Precision
1. Within assay variation (2 samples, 8 fold assay) Average C.V. is 2.28%
2. Reproducibility (3 samples, triplicates assay, 4 days) C.V. is 0.07 ~ 3.46%
Detection Range
Assay range of the standard curve is 30-3000 pg/mL
According to the standard procedure where the samples are diluted 5 times, practical assay range is 150-15 000 pg/mL
Sensitivity
1.5 pg/mL
General Description
C-peptide is formed from pro-insulin and co-secreted with insulin. Measuring the amount of c-peptide is useful as an index of insulin secretion. The Mouse C-Peptide ELISA kit is a simple, precise, and sensitive ELISA sandwich assay for mouse c-peptide.

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References


Characterization of soil microbes associated with a grazing-tolerant grass species,Stipa breviflora, in the Inner Mongolian desert steppe

ECOLOGY AND EVOLUTION

Authors: Chen, Lingling; Saixi, Yala; Yi, Ru; Baoyin, Taogetao

Although soil microbial communities are central in ecosystem functioning, we know little of their characterization for those associated with grazing-tolerant host plant species in grassland ecosystems in response to grazing. In this study, we used a high-throughput sequencing approach to characterize soil microbes from the rhizosphere and bulk soil of grazing-tolerant grass species,Stipa breviflora, in the Inner Mongolian desert steppe. We found that response mechanisms of soil bacteria distinct from fungal communities, and variance also occur between the rhizosphere and bulk soil communities under long-term grazing. Soil fungal communities and the co-occurrence networks inS. breviflorarhizosphere were more sensitive to long-term grazing than bacteria. We reveal that rhizosphere effects and soil water content were the main drivers of the changes in fungal communities and their co-occurrence networks. Moreover, the dominant bacterial phyla Bacteroidetes and Proteobacteria and fungal phyla Ascomycota and Glomeromycota might participate in regulating processes ofS. breviflora'sresponse to grazing. Overall, these findings give new snapshots of mechanisms of how grazing affects soil microbial communities, in an attempt to contribute to a clearer understanding of grazing-tolerant mechanism ofS. breviflora.

A modified velocity updating algorithm for a strapdown INS

MEASUREMENT SCIENCE AND TECHNOLOGY

Authors: Ben, Yueyang; Gao, Qianqian; Li, Qian; Liu, Xingyu

Velocity updating typically involves integration of the transformed specific force and gravity Coriolis velocity increments. If the angular rate and specific force vectors maintain fixed directions relative to a frame, updating the velocity in that frame will be straightforward. In practice, the coning angular rate and sculling specific force vectors cause a calculation error in velocity updating on the navigation frame. Thus, the development of sculling compensation algorithms for the reduction of the sculling error is necessary. In this study, a new method for the propagation of vehicle velocity is proposed. This method utilizes an auxiliary frame that slews along with the angular rate and specific force vectors, which remain fixed in the auxiliary frame. This transforms and integrates the specific force vector in this auxiliary frame such that it is error-free. Additionally, the updated velocity can be obtained by translating the above results to the navigation frame, thereby avoiding the need for sculling correction. The validity of this proposed method under the influence of a typical slewing motion was established experimentally, suggesting that it provides an efficient and accurate velocity propagation method that can be employed in aerospace applications.

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