Mouse Anti-Scl-70 ELISA Kit

Sample

Serum and Plasma

Species Reactivity

Mouse

Detection Method

iELISA

Intended Use

Anti-Scl-70 ELISA kit measures anti-Scl-70 antibodies in the serum.

Contents of Kit

1. 8 × 12 96-well plate coated with Scl-70 (4°C).
2. Anti-Mouse IgG antibody conjugated to HRP (4°C).
3. Mouse Scl-70 Positive Control/Standard (4°C).
4. 1× Diluent buffer (4°C).
5. 5× Assay wash buffer (4°C).
6. Substrate (4°C).
7. Stop Solution (4°C).

Storage

Store all reagents at 2-8°C.
All reagents must be brought to room temperature (20-25°C) prior to use.
When stored at 2-8°C, the diluted Assay wash buffer is stable until the kit expiration date.

General Description

Antibodies to Scl-70 are a specific immunological marker for scleroderma (or progressive systemic sclerosis, PSS), a systemic autoimmune disease characterized by collagen deposition and connective tissue destruction of the skin, blood vessels and certain internal organs. Studies have shown varying frequencies of Scl-70 antibodies in PSS. This antibody was found in approximately 20% of PSS patients in early studies but 75% in later studies. Scl-70 antibodies are directed against DNA-topoisomerase I which locates in the nucleus. The whole molecule of DNAtopoisomerase is 110 kDa but it is easily degraded by proteases to 100 kDa, 87 kDa and 70 kDa (Scl-70). PSS is classified into two types; diffuse scleroderma and limited scleroderma. Scl-70 antibodies are present specifically in diffuse scleroderma and centromere antibodies are present in limited scleroderma. Rarely, Scl-70 antibodies are found in SLE and MCTD patients.

Standard Curve

General Notes

Antibodies to Scl-70 are a specific immunological marker for scleroderma (or progressive systemic sclerosis, PSS), a systemic autoimmune disease characterized by collagen deposition and connective tissue destruction of the skin, blood vessels and certain internal organs. Studies have shown varying frequencies of Scl-70 antibodies in PSS. This antibody was found in approximately 20% of PSS patients in early studies but 75% in later studies. Scl-70 antibodies are directed against DNA-topoisomerase I which locates in the nucleus. The whole molecule of DNAtopoisomerase is 110 kDa but it is easily degraded by proteases to 100 kDa, 87 kDa and 70 kDa (Scl-70). PSS is classified into two types; diffuse scleroderma and limited scleroderma. Scl-70 antibodies are present specifically in diffuse scleroderma and centromere antibodies are present in limited scleroderma. Rarely, Scl-70 antibodies are found in SLE and MCTD patients.
Anti-Scl-70 ELISA kit measures anti-Scl-70 antibodies in the serum.