Mouse Anti-SSB (La) ELISA Kit

Name: Mouse Anti-SSB (La) ELISA Kit
SKU: DEIA116J
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, Plasma

Species Reactivity

Mouse

Detection Method

iELISA

Intended Use

Mouse Anti-SSB(La) ELISA kit is an assay for the quantitative measurement of mouseanti-SSB(La) antibody in samples. This product is intended for research use only.

Contents of Kit

1. Mouse SSB (La) pre-coated 96-microwell plate (One plate):
Store at 4°C until required
Ready to use
2. Anti-mouse IgG conjugated to HRP (10 μl):
Store at 4°Cuntil required
Dilute with 1× Diluent Buffer at 1:1000 before use
3. Mouse SSB (La) Standard 50 μg/ml (10 μl):
Store at 4°C until required
Dilute with 1× Diluent Buffer for standard curve
4. 1× Diluent Buffer (40 ml):
Store at 4°C until required
Ready to use
5. 5× Assay Wash Buffer (40 ml):
Store at 4°C until required
Dilute 40ml of 5× Assay Wash Buffer with 160 ml of dH₂O before use
6. Substrate (10 ml):
Store at 4°C until required
Check the Substrate to make sure it's clear. If its color has changed to dark blue, do not begin the experiment and contact us immediately.
7. Stop Solution (5 ml):
Store at 4°C until required
Ready to use
Caution: It is a strong acid substance. Therefore, be careful not to contact your skin and clothes with Stop solution and pay attention to disposal.

Storage

The shelf life ofthis product is 6 months. Use it before expiration.
Store all reagents at 2-8°C.
All reagents must be brought to room temperature (20- 25°C) prior to use.
When stored at 2-8°C, the diluted Assay wash buffer is stable until the kit expiration date.

General Description

The presence of antibodies against the SSB (also known as La) antigen has been advocated as a diagnostic marker for Sjogren's syndrome (SS), an autoimmune disease characterized by inflammation of the glands leading to diminished or absent glandular secretion. SS may present as a primary disease or associate with other systemic autoimmune diseases as secondary SS. The SSB antigen is a 47 kD ribonucleic protein associated with a spectrum of small RNAs and primarily resides in the nucleus. Antibodies to the SSB antigen appear in more than 80% of patients with primary or secondary SS. Anti-SSB antibodies usually co-present with anti-SSA antibodies, however due to more common of anti-SSA antibodies in other rheumatological conditions such as systemic lupus erythematosis (SLE) and mixed connective tissue disease (MCTD). It suggests that anti-SSB is more specific for primary and secondary SS than anti-SSA.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Optimization of methane production in a swine manure-rice straw anaerobic co-digestion process with sycamore sawdust biochar application

INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY

Authors: Jiang, B.; Lin, Y.; Lun, Y.; Xu, Z.

Abstract

Anaerobic co-digestion has been widely applied to re-utilize livestock manure; however, the low methane yield is still a key issue. Biochar could play an important role in anaerobic digestion. This study aims to produce and characterize sycamore sawdust biochar (SSB), determine the promoting effect on the swine manure-rice straw co-digestion system by applying the SSB, and obtain the optimal digesting conditions by using a Box-Behnken design of response surface methodology (RSM). The results showed that SSB had a porous structure composed of micropores and mesopores. The micropore surface area reached 162.87 m(2)/g, and the mesopore volume accounted for 43.67% of the total pore volume. SSB was also rich in C=O surface functional groups, and the BET specific surface area was up to 276.67 m(2)/g. Application of SSB could improve the pH value and the stability of the co-digestion system, and eventually improve the cumulative methane yield. The maximum cumulative methane yield in this test was 217.99 mL/(g VS) when 4% SSB was added. According to the response surface optimization analysis, the cumulative methane yield could be 247.20 mL/(g VS) under the optimal conditions: 94.54% swine manure, 8.78% TS and 5.49% SSB. Graphic abstract

Anti-Ro52 antibodies are a risk factor for interstitial lung disease in primary Sjogren syndrome

RESPIRATORY MEDICINE

Authors: Buvry, Charlotte; Cassagnes, Lucie; Tekath, Marielle; Artigues, Maxime; Pereira, Bruno; Rieu, Virginie; Le Guenno, Guillaume; Tournadre, Anne; Ruivard, Marc; Grobost, Vincent

Abstract

Objective: To determine whether anti-Ro52 antibodies are associated with ILD in pSS. Methods: Retrospective study based on the presence or absence of anti-Ro52 antibodies in patients with pSS. Patients underwent chest HRCT at the time of diagnosis or during follow-up. Results: Sixty-eight patients were included. Two groups were defined by the presence (n = 31) or absence (n = 37) of anti-Ro52 antibodies. ILD was significantly higher in the presence of anti-Ro52 (41.9%, n = 13) versus in the anti-Ro52-negative group (16.2%, n = 6; p = 0.019). Multivariate analysis adjusted for anti-SSA/Ro60, anti-SSB antibodies and rheumatoid factor status confirmed that anti-Ro52 antibodies positivity is a predictive factor for ILD (p = 0.01). Nonspecific interstitial pneumonia was the most common pattern of ILD (31.6%). The majority of patients were diagnosed with pSS simultaneously to ILD (52.6%). In the anti-Ro52-negative group, no patients develop ILD after 5 years of follow-up. Conclusion: In pSS, the risk of developing ILD is higher in the presence of anti-Ro52 antibodies. In patients with pSS and anti-Ro52 antibodies, a clinical screening and pulmonary functional tests with DLCO is necessary during the follow-up and should comprise chest HRCT if there is a decline in the DLCO or clinical symptoms or inspiratory crackles.