Mouse Adrb2 (Beta-2 adrenergic receptor) ELISA Kit

Name: Mouse Adrb2 (Beta-2 adrenergic receptor) ELISA Kit
SKU: DEIA-FN77
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

serum, plasma, cell culture supernatants, tissue homogenate

Species Reactivity

Mouse

Detection Method

Sandwich ELISA

Intended Use

For quantitative detection of Mouse Adrb2 (Beta-2 adrenergic receptor) in serum, plasma, tissue homogenates and other biological fluids.

Contents of Kit

1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Precision

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Detection Range

0.156-10 ng/mL

Sensitivity

0.094 ng/mL

Standard Curve

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Genetic Variation In Cftr Allele Mutations And Adrb2 Polymorphisms Influence Cardiac Hemodynamic Responses To Albuterol In Individuals With Cystic Fibrosis

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE

Authors: Van Iterson, E. H.; Baker, S. E.; Wheatley, C. M.; Morgan, W. J.; Snyder, E. M.

Abstract

Association of Beta 2 adrenergic receptor (Thr164Ile) polymorphism with Salbutamol refractoriness in severe asthmatics from Indian population

GENE

Authors: Bandaru, Srinivas; Tarigopula, Pramod; Akka, Jyothy; Marri, Vijaya Kumar; Kattamuri, Ramesh Kumar; Nayarisseri, Anuraj; Mangalarapu, Madhavi; Vinukonda, Swetha; Mundluru, Hema Prasad; Sagurthi, Someswar Rao

Abstract

Background: Thr164Ile polymorphism in the ADRB2 gene encoding beta 2 adrenergic receptor (beta(2)AR) has its functional consequence in declining ligand-receptor interactions and depressed coupling of beta(2)AR to adenylcyclase. In the present study, we sought to evaluate the possible association of Thr164Ile polymorphism with asthma susceptibility, pharmacogenetic response to Salbutamol and varying degrees of severity. Methods: Three hundred and ninety eight clinically diagnosed patients and four hundred and fifty six healthy controls were enrolled in the study. Patients were classified into severity classes according to the Global Initiative for Asthma (GINA) guidelines. To assess bronchodilator response, spirometry was performed before and 15 min after Salbutamol (200 mu g) delivery. Responders to Salbutamol were categorized if percentage reversibility was greater than or equal to 12% in them, while those showing reversibility <12% were classified as non responders. Further, responding phenotypes were stratified into severity groups. Genotyping was carried out by ARMS-PCR technique. Statistical methods were applied to test the significance of the results. Results: In the present study, polymorphism was not associated with disease susceptibility however; there was significant association with non-responding asthmatics. In case of severity subsets, the polymorphism was not associated with milder subtypes; although, notable association was observed with moderate and severe asthma subtypes. In addition, the polymorphism was significantly associated with non-responding patients with severe asthma. Conclusions: In south Indian population, the ADRB2 Thr164Ile polymorphism may not form susceptible variant to develop asthma, however, it can form a predictive maker for bronchodilator (Salbutamol) response in severe asthmatics. (C) 2016 Elsevier B.V. All rights reserved.