Mouse ADAMTS5 (A disintegrin and metalloproteinase with thrombospondin motifs 5) ELISA Kit (DEIA-FN46)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma, cell culture supernatants, tissue homogenate
Species Reactivity
Mouse
Intended Use
For quantitative detection of Mouse ADAMTS5 (A disintegrin and metalloproteinase with thrombospondin motifs 5) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
0.156-10 ng/mL
Sensitivity
0.094 ng/mL
Standard Curve

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References


Ullah, M; Sittinger, M; et al. Extracellular matrix of adipogenically differentiated mesenchymal stem cells reveals a network of collagen filaments, mostly interwoven by hexagonal structural units. MATRIX BIOLOGY 32:452-465(2013).
Larkin, I; et al. LINKING AN ADAMTS5-SPECIFIC THERAPEUTIC MONOCLONAL ANTIBODY TO A SENSITIVE BIOCHEMICAL MARKER OF TARGET ENGAGEMENT AND ACTIVITY FOR POTENTIAL APPLICATION AS A COMPANION DIAGNOSTIC. OSTEOARTHRITIS AND CARTILAGE 20:S290-S290(2012).

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