Anti-MYPT1 (aa 728 – 838) polyclonal antibody (CABT-BL6359)

Sheep Anti-Human MYPT1 (aa 728 – 838) polyclonal antibody for WB

Specifications


Host Species
Sheep
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
human MYPT1 (residues 728-838) [GST-tagged]
Conjugate
Unconjugated

Applications


Application Notes
WB: 1 µg/ml
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
PPP1R12A; protein phosphatase 1, regulatory subunit 12A; MBS; M130; MYPT1; protein phosphatase 1 regulatory subunit 12A
Entrez Gene ID
UniProt ID

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Downregulation of MYPT1 increases tumor resistance in ovarian cancer by targeting the Hippo pathway and increasing the stemness

MOLECULAR CANCER

Authors: Munoz-Galvan, Sandra; Felipe-Abrio, Blanca; Verdugo-Sivianes, Eva M.; Perez, Marco; Jimenez-Garcia, Manuel P.; Suarez-Martinez, Elisa; Estevez-Garcia, Purificacion; Carnero, Amancio

Background Ovarian cancer is one of the most common and malignant cancers, partly due to its late diagnosis and high recurrence. Chemotherapy resistance has been linked to poor prognosis and is believed to be linked to the cancer stem cell (CSC) pool. Therefore, elucidating the molecular mechanisms mediating therapy resistance is essential to finding new targets for therapy-resistant tumors. Methods shRNA depletion of MYPT1 in ovarian cancer cell lines, miRNA overexpression, RT-qPCR analysis, patient tumor samples, cell line- and tumorsphere-derived xenografts, in vitro and in vivo treatments, analysis of data from ovarian tumors in public transcriptomic patient databases and in-house patient cohorts. Results We show that MYPT1 (PPP1R12A), encoding myosin phosphatase target subunit 1, is downregulated in ovarian tumors, leading to reduced survival and increased tumorigenesis, as well as resistance to platinum-based therapy. Similarly, overexpression of miR-30b targeting MYPT1 results in enhanced CSC-like properties in ovarian tumor cells and is connected to the activation of the Hippo pathway. Inhibition of the Hippo pathway transcriptional co-activator YAP suppresses the resistance to platinum-based therapy induced by either low MYPT1 expression or miR-30b overexpression, both in vitro and in vivo. Conclusions Our work provides a functional link between the resistance to chemotherapy in ovarian tumors and the increase in the CSC pool that results from the activation of the Hippo pathway target genes upon MYPT1 downregulation. Combination therapy with cisplatin and YAP inhibitors suppresses MYPT1-induced resistance, demonstrating the possibility of using this treatment in patients with low MYPT1 expression, who are likely to be resistant to platinum-based therapy.

Oxytocin mediates ROCK dependent phosphorylation of the regulatory subunit (PPP1R12A) of myosin phosphatase to increase myosin phosphorylation in human myometrium

REPRODUCTIVE SCIENCES

Authors: Laney, J.; Lopez Bernal, A.

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