MST1 (Human) ELISA Kit (DEIA3957)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cell culture supernatant, plasma, serum, urine
Species Reactivity
Human
Intended Use
The MST1 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MST1 in serum, plasma, cell culture supernatants and urine.
Contents of Kit
1. MST1 Microplate
2. Wash Buffer Concentrate (20X)
3. Recombinant human MST1
4. Assay Diluent A
5. Assay Diluent B
6. Detection Antibody MST1
7. HRP-Streptavidin concentrate
8. TMB One-Step Substrate Reagent
9. Stop Solution
Storage
All kit components of this kit are stable at 2-8°C. For more detailed information, please download the following document on our website.
Sensitivity
8 pg/mL

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References


A ROR1 HER3 IncRNA signalling axis modulates the Hippo YAP pathway to regulate bone metastasis

NATURE CELL BIOLOGY

Authors: Li, Chunlai; Wang, Shouyu; Xing, Zhen; Lin, Aifu; Liang, Ke; Song, Jian; Hui, Qingsong; Yao, Jun; Chen, Zhongyuan; Park, Peter K.; Hawke, David H.; Zhou, Jianwei; Zhou, Yan; Zhang, Shuxing; Liang, Han; Hung, Mien-Chie; Gallick, Gary E.; Han, Leng; Lin, Chunru; Yang, Liuqing

Bone metastases remain a serious health concern because of limited therapeutic options. Here, we report that crosstalk between ROR1-HER3 and the Hippo-YAP pathway promotes breast cancer bone metastasis in a long noncoding RNA-dependent fashion. Mechanistically, the orphan receptor tyrosine kinase ROR1 phosphorylates HER3 at a previously unidentified site Tyr1307, following neuregulin stimulation, independently of other ErbB family members. p-HER3 Tyr1307 recruits the LLGL2-MAYA-NSUN6 RNA-protein complex to methylate Hippo/MST1 at Lys59. This methylation leads to MST1 inactivation and activation of YAP target genes in tumour cells, which elicits osteoclast differentiation and bone metastasis. Furthermore, increased ROR1, p-HER3 Tyr1307 and MAYA levels correlate with tumour metastasis and unfavourable outcomes. Our data provide insights into the mechanistic regulation and linkage of the ROR1-HER3 and Hippo-YAP pathway in a cancer-specific context, and also imply valuable therapeutic targets for bone metastasis and possible therapy-resistant tumours.

Inflammatory bowel disease (IBD) locus 12: is glutathione peroxidase-1 (GPX1) the relevant gene?

GENES AND IMMUNITY

Authors: Haeuser, F.; Rossmann, H.; Laubert-Reh, D.; Wild, P. S.; Zeller, T.; Mueller, C.; Neuwirth, S.; Blankenberg, S.; Lackner, K. J.

Genome-wide association studies have identified and repeatedly confirmed the association of rs3197999 in MST1 with inflammatory bowel disease (IBD). However, the underlying pathophysiology remains unclear. rs3197999 is a non-synonymous single-nucleotide polymorphism which modifies the function of macrophage stimulating protein-1 (MST1). We show by haplotyping that rs3197999 is in linkage disequilibrium with rs1050450 in GPX1, with almost complete cosegregation of the minor alleles. As shown by immunoassay, rs3197999 influences the MST-1 level in serum. But also rs1050450 causes an amino acid exchange in glutathione peroxidase 1 (GPx-1) and reduced activity of this antioxidant enzyme. The association of GPx deficiency and IBD in mice was already shown. We propose that GPx-1 is a better candidate than MST1 for the pathophysiologic link between IBD locus 12 and IBD.

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