MAPK3 (pT202/Y204)/MAPK1 (pT185/Y187) (Human) ELISA Kit (DEIA3904)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cell lysates, tissues lysates
Species Reactivity
Human
Intended Use
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-Erk1 (T202/Y204)/Erk2(T185/Y187).
Contents of Kit
1. Erk1/2Microplate
2. Wash Buffer Concentrate (20X)
3. Assay Diluent
4. Detection Antibody Erk1(T202/Y204)/Erk2(T185/Y187)
5. HRP-conjugated Anti-rabbit IgG
6. TMB One-Step Substrate Reagent
7. Stop Solution
8. Cell Lysate Buffer
9. Positive Control A431S002-1
Storage
Store the unopened kit at 4°C upon receipt and when it is not in use. For more detailed information, please download the following document on our website.

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References


NOP14 promotes proliferation and metastasis of pancreatic cancer cells

CANCER LETTERS

Authors: Zhou, Bin; Wu, Qiao; Chen, Ge; Zhang, Tai-Ping; Zhao, Yu-Pei

Pancreatic cancer (PC) is one of the most malignant cancers worldwide. We describe a novel gene, NOP14, which plays significant roles in PC cell proliferation and migration. Inhibition or overexpression of NOP14 expression in PC cells can reduce or promote motility, proliferation and metastatic capacity in vivo. In parallel, we observed changes in proteins related to migration, such as E-cadherin, vimentin, MMP9, RhoA and p53, along with proteins involved in proliferation, such as MAPK3 and CDK2. Taken together, our study provides new evidence for NOP14 in regulating PC cell proliferation and migration, and may provide new insights for clinical diagnosis and therapy. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

Glyphosate exposure induces inflammatory responses in the small intestine and alters gut microbial composition in rats

ENVIRONMENTAL POLLUTION

Authors: Tang, Qian; Tang, Juan; Ren, Xin; Li, Chunmei

Glyphosate is the most popular herbicide used worldwide. This study aimed to investigate the adverse effects of glyphosate on the small intestine and gut microbiota in rats. The rats were gavaged with 0, 5, 50, and 500 mg/kg of body weight glyphosate for 35 continuous days. The different segments of the small intestine were sampled to measure indicators of oxidative stress, ion concentrations and inflammatory responses, and fresh feces were collected for microbiota analysis. The results showed that glyphosate exposure decreased the ratio of villus height to crypt depth in the duodenum and jejunum. Decreased activity of antioxidant enzymes (T-SOD, GSH, GSH-Px) and elevated MDA content were observed in different segments of the small intestine. Furthermore, the concentrations of Fe, Cu, Zn and Mg were significantly decreased or increased. In addition, the mRNA expression levels of IL-1 beta, IL-6, TNF-alpha, MAPK3, NF-kappa B, and Caspase-3 were increased after glyphosate exposure. The 16 S rRNA gene sequencing results indicated that glyphosate exposure significantly increased alpha-diversity and altered bacterial composition. Glyphosate exposure significantly decreased the relative abundance of the phylum Firmicutes and the genus Lactobacillus, but several potentially pathogenic bacteria were enriched. In conclusion, this study provides important insight to reveal the negative influence of glyphosate exposure on the small intestine, and the altered microbial composition may play a vital role in the process. (C) 2020 Elsevier Ltd. All rights reserved.

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