Generated against taxol-stabalized microtubules from rat brain.
Conjugate
Unconjugated
Applications
Application Notes
Suitable for use in WB and IF. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Target
Alternative Names
MAK-2; DKFZp686I2148; Dendrite specific MAP; MAP 2; MAP-2; MAP2; MAP2_HUMAN; MAP2A; MAP2B; MAP2C; Microtubule associated protein 2; Microtubule-associated protein 2; Mtap 2
Citations
Publication ()
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Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells
Mária Tirpáková, Jaromír Vašíček, Andrea Svoradová , Andrej Baláži, Marián Tomka, Miroslav Bauer, Alexander Makarevich, Peter Chrenek
Applications: IF Reactive species: Rabbit
"Abstract: Although the rabbit is a frequently used biological model, the phenotype of rabbit adipose-derived mesenchymal stem cells (rAT-MSCs) is not well characterized. One of the reasons is the absence of specific anti-rabbit antibodies. The study aimed to characterize rAT-MSCs using flow cytometry and PCR methods, especially digital droplet PCR, which confirmed the expression of selected markers at the mRNA level. A combination of these methods validated the expression of MSCs markers (CD29, CD44, CD73, CD90 and CD105). In addition, cells were also positive for CD49f, vimentin, desmin, α-SMA, ALDH and also for the pluripotent markers: NANOG, OCT4 and SOX2. Moreover, the present study proved the ability of rAT-MSCs to differentiate into a neurogenic lineage based on the confirmed expression of neuronal markers ENO2 and MAP2. Obtained results suggest that rAT-MSCs have, despite the slight differences in marker expression, the similar phenotype as human AT-MSCs and possess the neurodifferentiation ability. Accordingly, rAT-MSCs should be subjected to further studies with potential application in veterinary medicine but also, in case of their cryopreservation, as a source of genetic information of endangered species stored in the gene bank." Article snippet: Cell culture was assessed after three days of induction of differentiation into neurogenic lineage: starting since overnight incubation with a MAP2 primary antibody (Clone BB7, Creative Diagnostics, Shirley, NY, USA) or ENO2 (*), followed by washing and consequent incubation with a goat anti-mouse IgG-FITC secondary antibody (*).
Figure 1. Immunofluorescence of selected surface markers of rabbit (rAT-MSCs) and human (hAT-MSCs) samples (scale bar = 100 µm).
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COA
Publication 
 monoclonal Antibody, clone FQS3472.jpg)
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