Detection Method
Sandwich-ELISA
Intended Use
The Leutenizing Horome (LH) ELISA is an enzyme immunoassay for the quantitative measurement of LH in urine. This test is used to detect the midcycle LH surge in urine, which is an aid in predicting the time of ovulation.
Contents of Kit
| No. | Components | Size | Storage Conditions |
| 1 | Pre-coated 96-Well Plate | 12×8 strips | 2-8°C |
| 2 | Standard | 1 mL × 6 | 2-8°C |
| 3 | Enzyme Conjugate | 11 mL × 1 | 2-8°C |
| 4 | Substrate Solution | 14 mL × 1 | 2-8°C |
| 5 | Stop Solution | 14 mL × 1 | 2-8°C |
| 6 | Wash Solution (40×) | 30 mL × 1 | 2-8°C |
Storage
When stored at 2 °C to 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
Opened reagents must be stored at 2 °C to 8 °C. Microtiter wells must be stored at 2 °C to 8 °C. Once the foil bag has been opened, care should be taken to close it tightly again. Opened kits retain activity for four weeks if stored as described above.
General Description
Luteinizing Hormone (LH) is a crucial heterodimeric glycoprotein hormone produced and secreted by the gonadotropic cells of the anterior pituitary gland, under the regulation of Gonadotropin-Releasing Hormone (Gn-RH) from the hypothalamus. In men (where it is also called ICSH), LH primarily stimulates the Leydig cells in the testes to produce testosterone. In women, LH plays a central role in the menstrual cycle, where a dramatic rise known as the "LH surge" triggers ovulation and initiates the formation of the corpus luteum, which subsequently secretes progesterone and estrogen. LH assays are routinely used in clinical settings, often alongside FSH, to evaluate pituitary and gonadal function, pinpoint ovulation, and diagnose conditions like menopause.
Citations
Publication ()
Have you cited DEIA2231 in a publication?
Let us know and earn a reward for your research.