This kit has been specifically developed and validated for use in detecting L. lactis HCPs as contaminants in products produced by recombinant expression in Lactococcus lactis. This kit utilizes antibodies raised against and affinity purified from HCPs derived from L. lactis spent culture media. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals.
* All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.
* The substrate reagent should not be used if its stopped absorbance at 450nm is greater than 0.1.
* Reconstituted wash solution is stable until the expiration date of the kit.
The lower limit of detection (LOD) is defined as that concentration corresponding to a signal two standard deviations above the mean of the zero standard. LOD in our laboratories was determined to be ~0.125 ng/mL.
The lower limit of quantitation (LOQ) is defined as the lowest concentration, where concentration coefficients of variation (CVs) are <20%. The LOQ is conservatively stated as <0.5 ng/mL in our laboratories.