Influenza B Hemagglutinin, HA ELISA Kit (CKERS-HA-245I)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Intended Use
The Influenza B HA ELISA kit is for the quantitative determination of Influenza B HA.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.
Contents of Kit
1. Bring all reagents to room temperature before use.
2. Capture Antibody: 0.5 mg/mL of mouse anti-Influenza B HA monoclonal antibody. Dilute to a working concentration of 1 μg/mL in CBS before coating.
3. Detection Antibody: 0.5 mg/mL of rabbit anti-Influenza B HA polyclonal antibody conjugated to horseradish-perosidase(HRP). Dilute to a working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
4. Standard: Each vial contains 20 ng of recombinant Influenza B HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C to -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.
Detection Antibody should be protected from prolonged exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.
The minimum detectable dose of Influenza B Influenza B HA was determined to be approximately 62.5 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.


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Potential Effect of Irrigation Intervals and Potassium Phthalate on Fennel Plants Grown in Semi-Arid Regions


Authors: Ali, Hanan; Ahmed, Nahlaa; Abu-hashim, M. S.

INCREASING competition on water resources between the agriculture sector and other sectors requires new irrigation regimes to perform relevant levels of production in semi-arid regions. Field experiment was conducted during two successive seasons at the Experimental Farm of EL-Quassassin, Ismailia Governorate, Egypt. This experiment was prepared to assess the effects of different irrigation intervals, potassium phthalate and their combinations on the growth, number of umbels, fruit yield,water use efficiency, and volatile oil production of fennel plant during two successive seasons 2016/2017 and 2017/2018, respectively. Three irrigation treatments, main factor, as follow: I-1 (every three days, which is the common in this region with 1150 m(3)/ha), I-2 (every five days with 766 m(3)/ha), and I-3 (every seven days with 383 m(3)/ha), and the potassium phthalate as subsidiary factor (0, 400,500, and 600 ppm) were applied. The results showed that, the highest vegetative growth parameters were related to the potassium phthalate at the rate of 600 ppm and irrigation interval 3 days.The highest values of fruit yield, as well as the volatile oil production were significantly affected by the potassium phthalate and irrigation frequency in both seasons. Nevertheless, the concentrations of the macro and micro-nutrients in fruits revealed an increasing trend with increasing the irrigation intervals.In addition, the lower the amount of water use, the higher the water use efficiency.The results of GLC analysis of fennel volatile oil indicated that methyl chavicol as the main component varied between (63.02 and 80.82%). The treatment of potassium phthalate at 600 ppm with irrigation every 3 days resulted in the lowest methyl chavicol percentage.Thus, the proper irrigation interval increases the plant water stress tolerance by increasing the water use efficiency and the nutrient concentrations of fruits.

Background nitrogen deposition controls the effects of experimental nitrogen addition on soil gross N transformations in forest ecosystems


Authors: Cheng, Yi; Wang, Jing; Ge, Zhiwei; Zhang, Jinbo; Cai, Yanjiang; Chang, Scott X.; Cai, Zucong; Chen, Han Y. H.

Nitrogen (N) deposition can profoundly alter soil N transformation processes and the long-term productivity of forest ecosystems. The response of soil gross N transformations to N deposition in forest ecosystems has been well studied through simulated N addition experiments. Simulated N addition experiments are conducted under a wide range of background N deposition rates. However, it remains unclear whether the response of soil gross N transformation rates to simulated N addition is dependent on background N deposition rates. Here, we collate results from the literature in forest ecosystems, and found, for the first time, that the responses of gross rates of N mineralization, nitrification, and NO3- immobilization to experimental N addition changed from positive to negative with increasing background N deposition rates with the thresholds for such changes were 3.23, 6.02, 1.90 kg N ha(- 1) yr(- 1), respectively. Our results suggest that background N deposition rates shall be incorporated into ecosystem models to better predict forest ecosystem N cycling under future N deposition scenarios.

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