The Influenza B HA ELISA kit is for the quantitative determination of Influenza B HA.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.
Contents of Kit
1. Bring all reagents to room temperature before use.
2. Capture Antibody: 0.5 mg/mL of mouse anti-Influenza B HA monoclonal antibody. Dilute to a working concentration of 1 μg/mL in CBS before coating.
3. Detection Antibody: 0.5 mg/mL of rabbit anti-Influenza B HA polyclonal antibody conjugated to horseradish-perosidase(HRP). Dilute to a working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
4. Standard: Each vial contains 20 ng of recombinant Influenza B HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C to -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.
Detection Antibody should be protected from prolonged exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.
The minimum detectable dose of Influenza B Influenza B HA was determined to be approximately 62.5 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.