This Ileitis Antibody ELISA is a blocking ELISA for the detection of exposure to Lawsonia (L.) intracellularis in pigs. This assay is successfully applied worldwide for determining epidemiological status on farm level and for proper scheduling of preventative and control measures.
The CD Ileitis Antibody ELISA showed good agreement with commonly used direct and indirect methods for detecting L. intracellularis infection. The IFAT and CD Ileitis Antibody ELISA results correlated well with the duration of L. intracellularis shedding
Ileitis is a wide spread enteric disease caused by the intracellular bacteria L. intracellularis. Epidemiology is strongly influenced by farm characteristics, thus showing big variations in time of onset, severity and prevalence of infection. The two major forms of Ileitis are a subclinical disease of chronic diarrhea leading to poor performance in pigs or an acute disease resulting in severe diarrhea with high mortality. Both forms result in severe financial concerns.
Preventing or reducing incidence and severity of clinical disease as well performance improvements are major goals in controlling Ileitis. Due to human health concerns the use of antibiotics has become more and more under scrutiny in recent years. Therefore control and prevention of Ileitis through vaccination, management improvements and biosecurity has gained importance. Preventative methods shall be scheduled prior to anticipated onset of relevant infection with L. intracellularis. Serology is an effective tool for confirming exposure therefore overcoming the limitations of detecting L. intracellularis in fecal samples.