Anti-IgG4 monoclonal antibody, Biotin (CABT-BL8638)

Rabbit Anti-Human IgG4 monoclonal antibody, Biotin for IHC, FC, ICC, ELISA, WB Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
SN230
Species Reactivity
Human
Immunogen
Peptide corresponding to the hinge region of Human IgG4
Conjugate
Biotin

Applications


Application Notes
ELISA: 50ng/well-200ng/well (for Capture);0.05ug/mL-0.2ug/mL (for Detection);
Western Blot (WB): 0.5 ug/mL-2 ug/mL;
Immunocytochemistry (ICC): 1 ug/mL-10ug/mL;
Immunohistochemistry (IHC): 1 ug/mL-10ug/mL.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
IGHG4; immunoglobulin heavy constant gamma 4 (G4m marker)

Citations


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References


Vivarelli, M; Emma, F; et al. Genetic homogeneity but IgG subclass-dependent clinical variability of alloimmune membranous nephropathy with anti-neutral endopeptidase antibodies. KIDNEY INTERNATIONAL 87:602-609(2015).
Silva, JP; Vetterlein, O; et al. The S228P Mutation Prevents in Vivo and in Vitro IgG4 Fab-arm Exchange as Demonstrated using a Combination of Novel Quantitative Immunoassays and Physiological Matrix Preparation. JOURNAL OF BIOLOGICAL CHEMISTRY 290:5462-5469(2015).

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