IgG Screen Nutritional ELISA Kit (DEIA301)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma
Species Reactivity
Human
Intended Use
The IgG Screen Nutritional 24 ELISA test kit has been designed for the detection and the quantitative determination of specific food antigen-related IgG antibodies in serum and plasma.
Contents of Kit
1. Microtiter Strips
2. Standards
3. Low positive Control
4. High positive Control
5. Sample Diluent
6. Conjugate
7. Substrate
8. Stop Solution
9. Washing Buffer
10. Plastic Foils
11. Instruction Booklet
12. Distribution Scheme
Storage
Stored at 2-8°C. Expiry data are found on the labels of the bottles and the outer package. For more detailed information, please download the following document on our website.

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References


Long-term effects of intensive B cell depletion therapy in severe cases of IgG4-related disease with renal involvement

IMMUNOLOGIC RESEARCH

Authors: Quattrocchio, Giacomo; Barreca, Antonella; Demarchi, Andrea; Fenoglio, Roberta; Ferro, Michela; Del Vecchio, Giulio; Massara, Carlo; Rollino, Cristiana; Sciascia, Savino; Roccatello, Dario

IgG4-related disease (IgG4-RD) is an immune-mediated disorder often showing elevated serum IgG4 concentrations, dense T and B lymphocyte infiltration, and IgG4-positive plasma cells and storiform fibrosis. We prospectively evaluated for 4 years 5 patients with histologically proven IgG4-RD of whom 3 had tubulointerstitial nephritis (TIN) and 2 had retroperitoneal fibrosis (RPF). They received an intensive B depletion therapy with rituximab. The estimated glomerular filtration rate of TIN patients after 1 year increased from 9 to 24 ml/min per 1.73 m2. IgG/IgG4 dropped from 3236/665 to 706/51 mg/dl, C3/C4 went up from 49/6 to 99/27 mg/dl, and the IgG4-RD responder index fell from 10 to 1. CD20(+) B cells decreased from 8.7 to 0.5%. A striking drop in interstitial plasma cell infiltrate as well as normalization of IgG4/IgG-positive plasma cells was observed at repeat biopsy. Both clinical and immunological improvement persisted over a 4-year follow-up. Treating these patients who were affected by aggressive IgG4-RD with renal involvement in an effort to induce a prolonged B cells depletion with IgG4 and cytokine production decrease resulted in a considerable rise in eGFR, with IgG4-RD RI normalization and a noteworthy improvement in clinical and histological features. Furthermore, the TIN subgroup was shown not to need for any maintenance therapy.

Glycosylation Alterations in Multiple Sclerosis Show Increased Proinflammatory Potential

BIOMEDICINES

Authors: Cvetko, Ana; Kifer, Domagoj; Gornik, Olga; Klaric, Lucija; Visser, Elizabeth; Lauc, Gordan; Wilson, James F.; Stambuk, Tamara

Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology. Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis. We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls. Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 x 10(-3)) and abundance of high-mannose structures (p = 1.48 x 10(-2)) were the most prominently altered IgG glycosylation traits. Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 x 10(-2)-4.28 x 10(-2)). Classification performance of N-glycans was examined by ROC curve analysis, resulting in an AUC of 0.852 for the total plasma N-glycome and 0.798 for IgG N-glycome prediction models. Our results indicate that multiple aspects of protein glycosylation are altered in MS, showing increased proinflammatory potential. N-glycan alterations showed substantial value in classification of the disease status, nonetheless, additional studies are warranted to explore their exact role in MS development and utility as biomarkers.

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