Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The ITGA2 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Alternative Names
ITGA2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2; integrin alpha-2; collagen receptor; platelet antigen Br; platelet glycoprotein GPIa; VLA2 receptor, alpha-2 subunit; CD49 antigen-like family member B; platelet membrane glycoprotein Ia; human platelet alloantigen system 5; very late activation protein 2 receptor, alpha-2 subunit;
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Concentration
The protein concentration was determined with BCA assay.
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
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Zhang, HL; Guo, FG; et al. Involvement of Host Cell Integrin alpha 2 in Cryptosporidium parvum Infection. INFECTION AND IMMUNITY 80:1753-1758(2012).
Kunicki, TJ; Williams, SA; et al. Mean Platelet Volume and Integrin Alleles Correlate With Levels of Integrins alpha(IIb)beta(3) and alpha(2)beta(1) in Acute Coronary Syndrome Patients and Normal Subjects. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 32:147-U337(2012).
COA
Certificate of Analysis
