Anti-IQGAP1 monoclonal antibody (DMABT-H14055)

Mouse anti-Human IQGAP1 monoclonal antibody for WB, IHC, IF, sELISA, ELISA Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
3D6
Species Reactivity
Human
Immunogen
IQGAP1 (NP_003861, 611 a.a. ~ 710 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Unconjugated

Target


Alternative Names
IQGAP1; IQ motif containing GTPase activating protein 1; ras GTPase-activating-like protein IQGAP1; HUMORFA01; KIAA0051; p195
Entrez Gene ID
UniProt ID

Product Background


Gene summary
IQGAP1 (IQ Motif Containing GTPase Activating Protein 1) is a Protein Coding gene. Diseases associated with IQGAP1 include bullous skin disease and adenomatous polyposis coli. Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics and Signaling by GPCR. GO annotations related to this gene include calcium ion binding and GTPase activator activity. An important paralog of this gene is IQGAP3. This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.
Antigen Description
Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. Ras GTPase-activating-like protein IQGAP1 (IQGAP1) also known as p195 is a ubiquitously expressed protein that in humans is encoded by the IQGAP1 gene. IQGAP1 is a scaffold protein involved in regulating various cellular processes ranging from organization of the actin cytoskeleton, transcription, and cellular adhesion to regulating the cell cycle. This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton such as the formin Dia1 (mDia1), with cell adhesion molecules (CAMs), and with several signaling molecules to regulate cell morphology and motility. For example, IQGAP1 expression is necessary for neuronal process outgrowth on the cell adhesion molecule PTPmu (PTPRM). Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines and its over-expression and distinct membrane localisation is also observed in a range of tumours. The function about IQGAP1 antigen include GTPase activator activity; GTPase inhibitor activity; Rac GTPase binding; Ras GTPase activator activity; calmodulin binding; protein binding; protein phosphatase binding.
Pathway
Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; C-MYB transcription factor network, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; E-cadherin signaling in the nascent adherens junction, organism-specific biosystem; ErbB1 downstream signaling, organism-specific biosystem.

Citations


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References


Berryman, M; Bretscher, A; et al. Identification of a novel member of the chloride intracellular channel gene family (CLIC5) that associates with the actin cytoskeleton of placental microvilli. MOLECULAR BIOLOGY OF THE CELL 11:1509-1521(2000).
Jacquemet, G; Morgan, MR; et al. Rac1 is deactivated at integrin activation sites through an IQGAP1-filamin-A-RacGAP1 pathway. JOURNAL OF CELL SCIENCE 126:4121-4135(2013).

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