Having trouble finding a right antibody for IHC? We provide hybridoma screening service with IHC [as well as ICC] using paraffin-embedded slides (but not cryosections) or tissue microarrays at extra charges. Customer may provide the slides or Tissue Arrays; otherwise, we may procure the slides or arrays at customers’ expense.
In IHC, it is not easy to determine the specificity of an antibody, since a perfect control is usually not available, except in the cases where there is a gene-knock out tissue to be used as a negative control [or a specific transgenic fusion protein can serve as a reference staining]. For this reason, an independent assays, such as ELISA, Western Blotting or IP are required to confirm the specific binding of the antibody to the target. Then the antibody can be tested in IHC assays; the staining must be specific, i.e. positive not to all tissues or cells and the staining is significantly stronger than the control isotype-matched negative control antibodies. Therefore, to obtain IHC-positive antibodies, we first use ELISA, Western Blotting [for denatured epitopes] and/or Immuno-precipitation [for native epitopes] to select the positive binders. From our experience, the more ELISA/Western/IP positive antibodies obtained, the better chance to identify an IHC positive antibody.