IFA: Incomplete Freund's adjuvant Adjuvants

Product Name

IFA: Incomplete Freund's adjuvant

Cat. No.

ADJU-013CL

Content

IFA (Incomplete Freund's Adjuvant) is provided as a ready-to-use, clear, pale yellow liquid. IFA is available in two quantities: 10 ml; 6 x 10 ml. Each 10 ml IFA vial contains 1.5 ml Mannide Monooleate and 8.5 ml Paraffin oil.

Storage and Stability

IFA is shipped at room temperature and should be stored at 4˚C.IFA is stable for 2 years when properly stored. DO NOT FREEZE.

Description

IFA (Incomplete Freund's adjuvant), a water-in-oil emulsion, is one of the most commonly used adjuvants in research. It is prepared from non-metabolizable oils (paraffin oil and mannide monooleate). IFA does not contain killed Mycobacterium tuberculosis found in Complete Freund's Adjuvant (CFA) and is thus less inflammatory. IFA induces a predominantly Th2-biased response through the formation of a depot at the injection site and the stimulation of antibody producing plasma cells. It has been suggested that NOD2 modulates the adjuvant effects of IFA. IFA is utilized to produce water-in-oil emulsions of antigens. It is routinely used for boosting immunizations subsequent to CFA. It can also be used for the initial immunization, particularly when a strong antigen is chosen or moderate antibody levels are sufficient.

Preparation of Antigen-IFA Emulsions

Antigens are preferentially diluted in saline or phosphate buffers. The amount of protein or conjugated peptide used for the primary immunization can be adjusted depending upon availability and immunogenicity of the antigen. For example, mice can be injected subcutaneously (s.c.) with 1 to10 μg of endotoxin-free ovalbumin.

Mix equal volumes of antigen and IFA using a 21 gauge needle andsyringe. A white emulsion will form immediately.
Mix vigourously for several minutes to form an emulsion withmaximum stability.
Note: A stable emulsion is essential to produce high titers of antibodies.
The volume of injection depends of the site of administration.
Vortex just before use.

The volume of injection depends of the site of administration.

Note: To avoid anaphylaxis, do not use adjuvants for intravenous injection.

Recommended maximum volumes for injection of antigen/adjuvant mixtures per site of injection for laboratory animals. (Reference: Lindblad EB., 2000. Freund's Adjuvants. In: Vaccine adjuvants: Preparation Methods and Research Protocols. Humana Press.Totowa, NJ).

IFA: Incomplete Freund's adjuvant