A DNA sequence encoding the Influenza A virus (A/Hangzhou/1/2013 (H7N9)) (AGI60301.1) hemagglutinin (Met1-Val524) was expressed with a C-terminal polyhistidine tag.
Nature
Recombinant
Tag/Conjugate
His
Molecular Weight
The recombinant hemagglutinin of Influenza A virus (A/Hangzhou/1/2013(H7N9)) comprises 517 amino acids and has a predicted molecular mass of 57.6 kDa. The apparent molecular mass of the protein is approximately 58-69 kDa in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Format
Lyophilized from sterile PBS, pH 7.4.1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -70°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Keywords
Influenzavirus A; Influenza A virus; Influenza A virus H7N9 HA;H7N9 HA; IAV H7N9 HA
Citations
Publication ()
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