Hyaluronic Acid ELISA Kit

Sample

biological fluids; cell supernatants

Detection Method

sELISA

Intended Use

The Hyaluronic Acid ELISA Kit is for the quantification of HA in low volume (25 μL) samples with HA> 130 kDa.

Contents of Kit

1.Hyaluronic Acid Detection Plate, 1 plate
2.Hyaluronic Acid Detector, 1 bottle
3.HA Standard (1.6 ug), 1 vial
4.10X Assay Buffer (10 mL), 1 bottle
5.TBS Tablets, 5 tablets
6.TMB Solution (12 mL), 1 bottle
7.1N H₂SO₄ Stop Solution (10 mL), 1 bottle
8.96-Well Mixing Plate (yellow), 1 plate
9.Microtiter plate seal, 2 seals

Storage

Upon receipt store kit Part 1 at -20°C and Part 2 at 4°C. Allow the reagents to warm to room temperature before opening vials.

General Description

Hyaluronan (HA) is a linear polysaccharide comprised of a repeating disaccharide of N-acetylglucosamine and D-glucuronic acid. The major function of HA is to provide structural support of tissue as part of the extracellular matrix (ECM). Thus, HA is widely presented in connective tissue in higher animals. The size of HA varies from 100 kD to 10,000 kD and is responsible for different functions. In humans, free HA enters circulation through the lymph node, where 80% is degraded and recycled by the liver and the remaining 20% is metabolized in the kidneys and excreted through urine. Multiple studies have shown that high serum HA levels correlates with liver disease.

Standard Curve