Humanin High Sensitivity ELISA Kit (DEIA-XYZ89)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma
Species Reactivity
human
Intended Use
The Humanin EIA Kit is a competitive immunoassay for in vitro quantitative measurement of Humanin in human serum or plasma.
Contents of Kit
1. EIA buffer concentrate
2. 96-well immunoplate with acetate plate sealer
3. Anti serum (lyophilized powder)
4. Standard (1 ug lyophilized powder)
5. Biotinylated tracer (lyophilized powder)
6. Sreptavidin-HRP
7. TMB substrate solution
8. Stop solution
9. Standard diluent 8ml
10. Datasheet
11. Protocol
Storage
Lyophilized components and standard diluent at a constant -20°C, The remaining components should be stored in the refrigerator (2-4°C)
Performance Characteristics
AVERAGE IC50: 11 ng/ml
Detection Range
0-200 ng/ml
Standard Curve

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References


Effects of novel tacrine-related cholinesterase inhibitors in the reversal of 3-quinuclidinyl benzilate-induced cognitive deficit in rats-Is there a potential for Alzheimer's disease treatment?

NEUROSCIENCE LETTERS

Authors: Misik, Jan; Korabecny, Jan; Nepovimova, Eugenie; Kracmarova, Alzbeta; Kassa, Jiri

Inhibitors of cholinesterase are important drugs for therapy of Alzheimer's disease and the search for new modifications is extensive, including dual inhibitors or multi-target hybrid compounds. The aim of the present study was a preliminary evaluation of pro-cognitive effects of newly-developed 7-MEOTA-donepezil like hybrids (compounds no. 1 and 2) and N-alkylated tacrine derivatives (compounds no. 3 and 4) using an animal model of pharmacologically-induced cognitive deficit. Male Wistar rats were subjected to tests of learning and memory in a water maze and step-through passive avoidance task. Cognitive impairment was induced by 3-quinuclidinyl benzilate (QNB, 2 mg kg(-1)), administered intraperitoneally 1 h before training sessions. Cholinesterase inhibitors were administered as a single therapeutic dose following the QNB at 30 min at the following dose rates; 1 (25.6 mg kg(-1)), 2 (12.3 mg kg(-1)), 3 (5.7 mg kg(-1)), 4 (5.2 mg kg(-1)). The decrease in total path within the 10-swim session (water maze), the preference for target quadrant (water maze) and the entrance latency (passive avoidance) were taken as indicators of learning ability in rats. The effects of novel compounds were compared to that of standards tacrine (5.2 mg kg(-1)) and donepezil (2.65 mg kg(-1)). QNB significantly impaired spatial navigation as well as fear learning. Generally, the performance of rats was improved when treated with novel inhibitors and this effect reached efficiency of standard donepezil at selected doses. There was a significant improvement in the groups treated with compounds 2 and 3 in all behavioral tasks. The rest of the novel compounds succeed in the passive avoidance test. In summary, the potential of novel inhibitors (especially compounds 2 and 3) was proved and further detailed evaluation of these compounds as potential drugs for Alzheimer's disease treatment is proposed. (c) 2015 Elsevier Ireland Ltd. All rights reserved.

Structure dependency of a 24-residue peptide humanin on solvent and preferential solvation by trifluoroethanol studied by multicanonical sampling

CHEMICAL PHYSICS LETTERS

Authors: Yagisawa, Ryosuke; Kamiya, Narutoshi; Ikebe, Jinzen; Umezawa, Koji; Higo, Junichi

We performed an enhanced conformation sampling, multicanonical molecular dynamics simulation, of humanin in two solvents: pure water and 30% 2,2,2-trifluoroethanol (TFE)/water co-solvent. Computed helix content at 280 K was 4% in water and 25% in TFE/water. In both solvents, computed free-energy landscape consisted of clearly distinguishable clusters at 280 K. However, the cluster positions in the landscape had no overlap between the water and TFE/water systems. The clusters in TFE/water involved various types of helical structures. Preferential solvation by TFE was seen around the helical regions of humanin. Hydrophobic residues and TFE molecules worked together to induce the helix formation. (c) 2008 Elsevier B. V. All rights reserved.

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