Detection Method
Indirect ELISA
Intended Use
The Human snRNP-C-Ab ELISA KIT is to be used for the quantitative detection of antibodies against the snRNP complex in human serum.
Contents of Kit
1. 5X Sample Buffer
2. 50X Wash Buffer
3. Negative Control
4. Positive Control
5. Calibrators
6. Conjugate
7. TMB Substrate
8. Stop Solution
9. Microtiterplate
Storage
Store all reagents and the microplate at 2-8°C, in their original containers. Once prepared, reconstituted solutions are stable for 1 month at 4°C, at least. Reagents and the microplate shall be used within the expiry date indicated on each component, only. Avoid intense exposure of TMB solution to light. Store microplates in designated foil, including the desiccant, and seal tightly.
Detection Range
0-300 U/mL
Citations
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Lauber, J; Plessel, G; et al. The human U4/U6 snRNP contains 60 and 90kD proteins that are structurally homologous to the yeast splicing factors Prp4p and Prp3p. RNA-A PUBLICATION OF THE RNA SOCIETY 3:926-941(1997).
MA, J; KING, N; et al. ANTIBODY PENETRATION OF VIABLE HUMAN-CELLS .2. ANTI-RNP ANTIBODIES BINDING TO RNP ANTIGEN EXPRESSED ON CELL-SURFACE, WHICH MAY MEDIATE THE ANTIBODY INTERNALIZATION. CLINICAL AND EXPERIMENTAL IMMUNOLOGY 93:396-404(1993).
COA
Certificate of Analysis
