Human sCD23 ELISA Kit (DEIA2053)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cell culture supernatants, serum, EDTA plasma, citrate plasma, heparin plasma
Species Reactivity
Human
Intended Use
The human sCD23 ELISA is an enzyme-linked immunosorbent assay for measurement of human sCD23.
Contents of Kit
1. 1 aluminium pouch with a Microwell Plate coated with monoclonal antibody to human sCD23
2. 1 vial ( 100 μL ) Biotin-Conjugate anti-human sCD23 monoclonal antibody
3. 1 vial ( 150 μL ) Streptavidin-HRP
4. 2 vials human sCD23 Standard lyophilized, 400 U/mL upon reconstitution
5. 1 vial Control high, lyophilized
6. 1 vial Control low, lyophilized
7. 1 vial ( 5 mL ) Assay Buffer Concentrate 20X ( PBS with 1% Tween 20 and 10% BSA )
8. 1 bottle ( 50 mL ) Wash Buffer Concentrate 20X ( PBS with 1% Tween 20 )
9. 1 vial ( 15 mL ) Substrate Solution ( tetramethyl-benzidine )
10. 1 vial ( 12 mL ) Stop Solution ( 1M Phosphoric acid )
11. 1 vial ( 0.4 mL ) Blue-Dye
12. 1 vial ( 0.4 mL ) Green-Dye
13. 1 vial ( 0.4 mL ) Red-Dye
Storage
Store kit reagents between 2-8°C. For more detailed information, please download the following document on our website.
Detection Range
12.5-400 U/mL
Sensitivity
6.789 U/mL

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References


The effects of T4 and A5/80 phages on the expression of immunologically important genes in differentiated Caco-2 cells

POSTEPY HIGIENY I MEDYCYNY DOSWIADCZALNEJ

Authors: Borysowski, Jan; Miedzybrodzki, Ryszard; Przybylski, Maciej; Owczarek, Barbara; Weber-Dabrowska, Beata; Gorski, Andrzej

Introduction: Bacteriophages are an abundant component of the mucosal microbiota in humans and some animal species. Intestinal epithelial cells (IECs) are the key element responsible for the induction and regulation of immune responses in the gut mucosa. The objective of this study was to evaluate the effects of T4 and A5/80 bacteriophages on the expression of immunologically important genes in Caco-2, a model cell line for IECs. Materials & Method: Bacteriophages were added to cultures of differentiated Caco-2 cells for 12 hours, while control cultures were treated with phosphate-buffered saline (PBS). Expression of genes in Caco-2 cells was determined using custom-made RT2 Profiler PCR Arrays, which allow for the evaluation of gene expression with the sensitivity and specificity of real-time PCR. We evaluated the expression of 21 genes which are important for the immune functions of IECs, including ILIB, IL6, IL7, IL10, IL15, IL18, IL25, IL33, TGFBI, TNF, CXCL8, CCL2, TSLP, FCER2, PIGR, DEFB4A, CAMP, REG3G, TNFSFI3, TNFSFI3B, and MUC2. Results: Both examined phages significantly influenced the expression of a number of genes compared with control cultures. In particular, T4 significantly increased the expression of the CCL2 and DEFB4A genes, while A5/80 induced the expression of the PIGR gene. Discussion: Together with the findings from previous studies, our results suggest that by modulating the expression of some genes, bacteriophages may affect immune responses in the gut mucosa.

Single-cell RNA-Seq of follicular lymphoma reveals malignant B-cell types and coexpression of T-cell immune checkpoints

BLOOD

Authors: Andor, Noemi; Simonds, Erin F.; Czerwinski, Debra K.; Chen, Jiamin; Grimes, Susan M.; Wood-Bouwens, Christina; Zheng, Grace X. Y.; Kubit, Matthew A.; Greer, Stephanie; Weiss, William A.; Levy, Ronald; Ji, Hanlee P.

Follicular lymphoma (FL) is a low-grade B-cell malignancy that transforms into a highly aggressive and lethal disease at a rate of 2% per year. Perfect isolation of the malignant B-cell population from a surgical biopsy is a significant challenge, masking important FL biology, such as immune checkpoint coexpression patterns. To resolve the underlying transcriptional networks of follicular B-cell lymphomas, we analyzed the transcriptomes of 34 188 cells derived from 6 primary FL tumors. For each tumor, we identified normal immune subpopulations and malignant B cells, based on gene expression. We used multicolor flow cytometry analysis of the same tumors to confirm our assignments of cellular lineages and validate our predictions of expressed proteins. Comparison of gene expression between matched malignant and normal B cells from the same patient revealed tumor-specific features. Malignant B cells exhibited restricted immunoglobulin (Ig) light chain expression (either Ig kappa or Ig lambda), as well the expected upregulation of the BCL2 gene, but also downregulation of the FCER2, CD52, and major histocompatibility complex class II genes. By analyzing thousands of individual cells per patient tumor, we identified the mosaic of malignant B-cell subclones that coexist within a FL and examined the characteristics of tumor-infiltrating T cells. We identified genes coexpressed with immune checkpoint molecules, such as CEBPA and B2M in regulatory T (Treg) cells, providing a better understanding of the gene networks involved in immune regulation. In summary, parallel measurement of single-cell expression in thousands of tumor cells and tumor-infiltrating lymphocytes can be used to obtain a systems-level view of the tumor microenvironment and identify new avenues for therapeutic development.

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