Human rhinovirus (HRV) 3C protease with GST and His tags expressed inEscherichia coli .
In 50 mM Tris-HCl, pH8.0, 150 mM NaCl, 1 mM EDTA, 1 mM TCEP, 50% glycerol
2-8°C short term, -20°C long term
Human rhinoviruses are the most common viral infective agents in humans and are the predominant cause of the common cold. Rhinovirus infection proliferates in temperatures between 33–35 °C (91–95 °F), and this may be why it occurs primarily in the nose. Rhinovirus is a species in the genus Enterovirus of the Picornaviridae family of viruses. There are 99 recognized types of Human rhinoviruses that differ according to their surface proteins. They are lytic in nature and are among the smallest viruses, with diameters of about only 30 nanometers. Other viruses such as smallpox and vaccinia are around 10 times larger at about 300 nanometers.
The primary route of entry for Human rhinoviruses is the upper respiratory tract. Afterward, the virus binds to ICAM-1 (Inter-Cellular Adhesion Molecule 1) also known as CD54 (Cluster of Differentiation 54) receptors on respiratory epithelial cells. As the virus replicates and spreads, infected cells release distress signals known as chemokines and cytokines (which in turn activate inflammatory mediators). Infection occurs rapidly, with the virus adhering to surface receptors within 15 minutes of entering the respiratory tract. Just over 50% of symptomatic individuals will experience symptoms within 2 days of infection. Only about 5% of cases will have an incubation period of less than 20 hours, and, on the other end, it is expected that 5% of cases would have an incubation period of greater than four and a half days.