Contents of Kit
1. Human Tetanus toxin Ab Coated Wells, 96-well polystyrene microplate (12 strips of 8 wells): 1 plate, Ready-to-use.
2. Human Tetanus IgG Standard 0 IU/mL: 1 mL/vial, 1 vial, Ready-to-use.
3. Human Tetanus IgG Standard 0.01 IU/mL: 1 mL/vial, 1 vial, Ready-to-use.
4. Human Tetanus IgG Standard 0.02 IU/mL: 1 mL/vial, 1 vial, Ready-to-use.
5. Human Tetanus IgG Standard 0.04 IU/mL: 1 mL/vial, 1 vial, Ready-to-use.
6. Human Tetanus IgG Standard 0.06 IU/mL: 1 mL/vial, 1 vial, Ready-to-use.
7. Human Tetanus IgG Standard 0.10 IU/mL: 1 mL/vial, 1 vial, Ready-to-use.
8. HRP-conjugate reagent: 1 bottle, 10 mL, Ready-to-use.
9. Sample Diluent A: 1 bottle, 10 mL, Ready-to-use.
10. Sample Diluent B: 1 bottle, 10 mL, Ready-to-use.
11. Dilution plate (uncoated plate), 96-well polystyrene microplate: 1 plate, Ready-to-use.
12. CHROMOGEN SOLUTION A: 1 vial, 6 mL, Ready-to-use.
13. CHROMOGEN SOLUTION B: 1 vial, 6 mL, Ready-to-use.
14. Wash Solution Concentrate (20×), 50 mL with preservatives: 1 bottle
16. Datasheet: 1
17. Plastic Bag: Resealable, for the dry storage of non-used strips
18. Plastic Foils: 3 pieces to cover the microtiter strips during the incubation
Storage
The components of the kit will remain stable (12 months) through the expiration date indicated on the label and package with desiccant when stored between 2-8°C, do not freeze. To assure maximum performance of this Tetanus toxin IgG ELISA kit, protect the reagents from contamination with microorganism or chemicals during storage.
Detection Range
Detection Range: 0.01IU/ml~0.1IU/ml
In order for an assay to be considered valid (R2 ≥ 0.98), the following criteria must be met:
Standard A (0 IU/ml): Absorbance value ≤ 0.10
Standard A (0.1 IU/ml): Absorbance value ≥ 0.80
If the sample OD is higher than the upper limit of standard curve, the sample should be re-diluted and the experiment rerun. Multiply the result by dilution factor when calculating the unknown.
Datasheet