Human Tetanus Toxoid antibody ELISA Kit (DEIA1026)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma
Species Reactivity
Human
Intended Use
This kit is an indirect enzyme-linked immunosorbent assay for quantitative detection of IgG antibodies to Tetanus Toxoid in human serum or plasma. It is intended for diagnosing and monitoring of patients related to infection by Tetanus Toxoid.
Contents of Kit
1. Human Tetanus toxin Ab Coated Wells, 96-well polystyrene microplate (12 strips of 8 wells): 1 plate, Ready-to-use.
2. Human Tetanus IgG Standard 0 IU/mL: 1 mL/vial, 1 vial, Ready-to-use.
3. Human Tetanus IgG Standard 0.01 IU/mL: 1 mL/vial, 1 vial, Ready-to-use.
4. Human Tetanus IgG Standard 0.02 IU/mL: 1 mL/vial, 1 vial, Ready-to-use.
5. Human Tetanus IgG Standard 0.04 IU/mL: 1 mL/vial, 1 vial, Ready-to-use.
6. Human Tetanus IgG Standard 0.06 IU/mL: 1 mL/vial, 1 vial, Ready-to-use.
7. Human Tetanus IgG Standard 0.10 IU/mL: 1 mL/vial, 1 vial, Ready-to-use.
8. HRP-conjugate reagent: 1 bottle, 10 mL, Ready-to-use.
9. Sample Diluent A: 1 bottle, 10 mL, Ready-to-use.
10. Sample Diluent B: 1 bottle, 10 mL, Ready-to-use.
11. Dilution plate (uncoated plate), 96-well polystyrene microplate: 1 plate, Ready-to-use.
12. CHROMOGEN SOLUTION A: 1 vial, 6 mL, Ready-to-use.
13. CHROMOGEN SOLUTION B: 1 vial, 6 mL, Ready-to-use.
14. Wash Solution Concentrate (20×), 50 mL with preservatives: 1 bottle
16. Datasheet: 1
17. Plastic Bag: Resealable, for the dry storage of non-used strips
18. Plastic Foils: 3 pieces to cover the microtiter strips during the incubation
Storage
The components of the kit will remain stable (12 months) through the expiration date indicated on the label and package with desiccant when stored between 2-8°C, do not freeze. To assure maximum performance of this Tetanus toxin IgG ELISA kit, protect the reagents from contamination with microorganism or chemicals during storage.
Detection Range
Detection Range: 0.01IU/ml~0.1IU/ml

In order for an assay to be considered valid (R2 ≥ 0.98), the following criteria must be met:
Standard A (0 IU/ml): Absorbance value ≤ 0.10
Standard A (0.1 IU/ml): Absorbance value ≥ 0.80

If the sample OD is higher than the upper limit of standard curve, the sample should be re-diluted and the experiment rerun. Multiply the result by dilution factor when calculating the unknown.
Standard Curve

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