Human Surfactant Protein A ELISA Kit

Sample

Serum, cell culture supernatant and plasma

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The Human Surfactant Protein A (SPA) ELISA kit is used as an analytical tool for quantitative determination of Human Surfactant Protein A, SPA in serum, plasma and cell culture supernatant.

Contents of Kit

1. IgG1 Antibody Coated Microtiter Plate (12 x 8 wells) - 1 no
2. Concentrated Standard, Human SPA (64ng/ml) - 0.5 ml
3. Biotinylated SPD Antibody - 1 ml
4. Streptavidin-HRP Conjugate - 6 ml
5. Standard Diluent - 3 ml
6. 25X Wash Buffer - 20 ml
7. Substrate A - 6 ml
8. Substrate B - 6 ml
9. Stop Solution - 6 ml

Storage

1. All reagents should be stored at 2°C to 8°C for stability.
2. All the reagents and wash solutions should be used within 12 months from manufacturing date.
3. Before using, bring all components to room temperature (18-25°C). Upon assay completion ensure all components of the kit are returned to appropriate storage conditions.
4. The Substrate is light-sensitive and should be protected from direct sunlight or UV sources.

Performance Characteristics

This kit has been validated. Please view the details herein below

Detection Range

2-32ng/ml

Sensitivity

0.217ng/ml

General Description

The ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.