serum, plasma, other biological fluids
This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). It is developed for quantitative measurement of human PMN Elastase in serum, plasma and other biological fluids.
Contents of Kit
1. Human PMN Elastase Microplate
2. Standard (freeze dried)
3. Biotin-antibody (100x)
4. HRP-avidin (100x)
5. Sample Diluent
6. Antibody Diluent
7. HRP-avidin Diluent
8. TMB Solution A
9. TMB Solution B
10. TMB Stop Solution
11. Wash Buffer (25x)
1. Unopened Kit: Store at 2-8°C. Do not use past kit expiration date.
2. Opened/Reconstituted Reagents: Unmixed TMB Solution A; Unmixed TMB Solution B; TMB Stop Solution; Wash Buffer; Sample Diluent; Antibody Diluent; HRP-avidin Diluent. The above mentioned reagents should be stored for up to 1 month at 2-8°C.
3. Microplate Wells: Return unused wells to the foil pouch con-taining the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2-8°C.
The minimum detectable dose of human PMN Elastase is typically less than 0.1 ng/mL.