Human L-Phenylalanine ELISA Kit

Sample

Plasma

Species Reactivity

Human

Detection Method

cELISA

Intended Use

Enzyme Immunoassay for the quantitative determination of L-Phenylalanine (PHE) in plasma samples. After extraction and derivatization L-Phenylalanine is quantitatively determined by ELISA.

Contents of Kit

1. Adhesive Foil - Ready to use. 1 x 4 foils
2. Reaction Plate - Ready to use. 1 x 96 well plate, empty in a resealable pouch.
3. Wash Buffer Concentrate - Concentrated 50x. Buffer with a non-ionic detergent and physiological pH. 1 x 20 mL/vial.
4. Enzyme Conjugate - Ready to use. Goat anti-rabbit immunoglobulins conjugated with peroxidase. 1 x 12 mL/vial.
5. Substrate - Ready to use. Chromogenic substrate containing tetramethylbenzidine, substrate buffer and hydrogen peroxide. 1 x 12 mL/black vial.
6. Stop Solution - Ready to use. 1 x 12 mL/vial.
7. Microtiter Strips - Ready to use. 1 x 96 well (12x8) antigen precoated microwell plate in a resealable pouch with desiccant, silver bag
8. L-Phenylalanine Antiserum - Ready to use. Rabbit anti-L-Phenylalanine antibody. 1 x 6 mL/vial.
9. Assay Buffer - Ready to use. Buffer with alkaline pH. 1 x 20 mL.
10. Equalizing Reagent - Lyophilized. 1 vial.
11. D-Reagent - Ready to use. Crosslinking agent in dimethylsulfoxide. 1 x 3 mL/vial.
12. Reducing Concentrate – Concentrated 100×. Reducing agent in sodium hydroxide. 1 x 1 mL/vial.
13. PBS - Ready to use. Phosphate Buffered Saline. 1 x 50 mL/vial.
14. Precipitating Reagent - Ready to use. Acidic reagent for precipitation of plasma/serum proteins. 2 x 4 mL/vial.
15. Standards and Controls - Ready to use

Storage

Store the unopened reagents at 2 - 8°C until expiration date. Do not use components beyond the expiry date indicated on the kit labels. Once opened the reagents are stable for 1 month when stored at 2 – 8°C. Once the resealable pouch has been opened, care should be taken to close it tightly with desiccant again.

Detection Limit

1.7 μM