Human Prostatic Acid Phosphatase (PAP) ELISA Kit

Sample

serum

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

Human Prostatic Acid Phosphatase (PAP) ELISA Kit is a quantitative solid phase enzyme-linked immuno-sorbent assay. This test provides quantitative measurement of human prostatic acid phosphatase (PAP) in serum.

Contents of Kit

1. Micro-well Strips
2. Enzyme Conjugate (11 mL)
3. Sample Diluent or Zero Standard (11 mL)
4. Reference Standard Set (0.75 mL/each)
5. TMB Solution (11 mL)
6. Washing buffer concentrate 100x (10 mL)
7. Stop Solution
8. Well holder
9. Package Insert

Storage

1. Store the kits at 2-8°C in a refrigerator.
2. Keep micro-wells in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit. TMB Solution should be colorless; if the solution turns blue, it must be replaced.

General Description

The phosphatases include two main types. The alkaline phophatase has a pH optimum of about 9 while acid phosphatase (AcP) has its optimal activity at a pH of about 5. The AcP, first demonstrated in the urine in 1925, was found to be much more prevalent in male than in female urine. Prostatic acid phosphatase (PAP) enzyme activity was found to be localized in organs of the male genital tract. The presence of PAP in sera of patients with prostatic cancer was first demonstrated by Gutman. It was shown that PAP concentration was elevated in many man with primary prostatic carcinoma and metastatic lesions of the prostate.
The release of PAP into the blood stream by primary or metastatic carcinoma of the prostate may provide the clinician with a means of following remission or relapse of a prostatic malignancy. Therefore, sensitive and accurate measurement of serum PAP is essential in monitoring the effectiveness of various therapeutic treatments.
Because moderate elevation of serum PAP is observed to accompany many nonprostatic diseases, a specific assay for determination of PAP is established. The current methodologies for determination of PAP activity using the colorimetric methods and immunochemical methods have many limitations.