Human Osteocalcin ELISA Kit (CDN-OC-0251)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma, other biological fluids
Species Reactivity
Human
Intended Use
This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). It is developed for quantitative measurement of human osteocalcin in serum, plasma and other biological fluids.
Contents of Kit
1. Microwell plate: 1 x 96 wells
2. Standard (freeze dried): 2 vials
3. Biotin-antibody (100x): 1 x 120 μL
4. HRP-avidin (100x): 1 x 120 μL
5. Sample Diluent: 2 x 15 mL
6. Antibody Diluent: 1 x 12 mL
7. HRP-avidin Diluent: 1 x 12 mL
8. TMB Solution A: 1 x 10 mL
9. TMB Solution B: 1 x 1 mL
10. TMB Stop Solution: 1 x 12 mL
11. Wash Buffer (25X): 1 x 20 mL
Storage
Store component of the kit at 2-8°C. For more detailed information, please download the following document on our website.

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References


Arachidonic Acid: A Bridge between Traumatic Brain Injury and Fracture Healing

JOURNAL OF NEUROTRAUMA

Authors: Yang, Shuguang; Ma, Yanhong; Liu, Yong; Que, Haiping; Zhu, Changqiang; Liu, Shaojun

Traumatic brain injury (TBI) is associated with enhanced osteogenesis. The aim of this study was to investigate the effect of serum from TBI rats on fracture healing. Results from this study showed that the serum from TBI rats enhanced the expression of bone gamma carboxyglutamate protein (BGLAP), and promoted in vitro proliferation of MC3T3-E1 cells, a mouse osteoblastic cell line. Furthermore, gas chromatography/mass spectrometry (GC/MS) coupled with multivariate statistical analysis was used to identify the changes in global serum metabolites after TBI. We found that arachidonic acid (AA) was significantly enhanced in serum metabolites in TBI subjects, while hydroxybutyric acid, leucine, malic acid, 5-oxyproline, isocitric acid, mannose, and uric acid were reduced. Finally, we examined the effects of AA on BGLAP expression and cell proliferation in MC3T3-E1 cells. We found that BGLAP expression and proliferation of osteoblasts were positively regulated in the presence of AA. These findings suggest that the increased AA in serum after TBI may play a key role in enhancing the speed of fracture healing.

Effect of In Vitro Passaging on the Stem Cell-Related Properties of Tendon-Derived Stem Cells-Implications in Tissue Engineering

STEM CELLS AND DEVELOPMENT

Authors: Tan, Qi; Lui, Pauline Po Yee; Rui, Yun Feng

This study aimed to compare clonogenicity, proliferation, stem cell-related marker expression, senescence, and differentiation potential of rat patellar tendon-derived stem cells (TDSCs) at early (P5), mid (P10), and late (P20, P30) passages. The clonogenicity of the cells was assessed by colony-forming assay and their proliferative potential was assessed by bromodeoxyuridine assay. The surface expression of CD90 and CD73 was assessed by flow cytometry. The cellular senescence was assessed by beta-galactosidase activity. The adipogenic, chondrogenic, and osteogenic differentiation potentials of TDSCs were assessed by standard assays after induction. The mRNA expression of tendon-related markers, scleraxis (Scx) and tenomodulin (Tnmd), was measured by quantitative real-time reverse transcription-polymerase chain reaction. Both the colony numbers and proliferative potential of TDSCs increased with passaging. Concomitantly, there was significant upregulation of b-galactosidase activity with TDSC passaging. The subculture of TDSCs downregulated the expression of CD90 and CD73. Lipid droplets were formed in the early and mid passages of TDSCs upon adipogenic induction, but were absent in the late passages. The expression of peroxisome proliferator activator receptor gamma 2 (PPAR gamma 2) and CCAAT/enhancer binding protein alpha (C/EBP alpha) in TDSCs after adipogenic induction decreased with passaging. Chondrogenesis, proteoglycan deposition, collagen type II protein expression, collagen type 2A1 (Col2AI), and aggrecan (Acan) mRNA expression were less in pellets formed with later passages of TDSCs after chondrogenic induction. The expression of Scx and Tnmd was lower in the late, compared with early and mid, passages of TDSCs. However, matrix mineralization and expression of alkaline phosphatase (Alpl) and osteocalcin (Bglap) mRNA after osteogenic induction increased with TDSC passaging. Researchers and clinicians should consider the changes of stem cell-related properties of TDSCs when multiplying them in vitro for tissue engineering.

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