Human Nidogen-1 / Entactin ELISA Kit (DEIA5698)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma, cell culture supernatants, urine
Species Reactivity
Human
Intended Use
The Human Nidogen-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Nidogen-1 in serum, plasma, cell culture supernatants and urine.
Contents of Kit
1. Nidogen-1 Microplate: 96 wells (12 strips x 8 wells) coated with anti-human Nidogen-1.
2. Wash Buffer Concentrate (20X): 25 mL of 20X concentrated solution.
3. Standards: 2 vials, recombinant human Nidogen-1.
4. Assay Diluent: 15 mL of 5X concentrated buffer. For Standard/Sample (serum/plasma samples/cell culture medium/urine) diluent.
5. Detection Antibody Nidogen-1: 2 vial of biotinylated anti-human Nidogen-1.
6. HRP-Streptavidin concentrate: 200 μL 200X concentrated HRP-conjugated streptavidin.
7. TMB One-Step Substrate Reagent: 12 mL of 3, 3', 5, 5'-tetramethylbenzidine (TMB) in buffered solution.
8. Stop Solution: 8 mL of 0.2 M sulfuric acid.
Storage
May be stored for up to 6 months at 2-8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be stored for up to 1 month at 2-8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. For more detailed information, please download the following document on our website.
Sensitivity
0.1 ng/mL

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References


Vasudevan, A; Ho, MSP; et al. Basement Membrane Protein Nidogen-1 Shapes Hippocampal Synaptic Plasticity and Excitability. HIPPOCAMPUS 20:608-620(2010).
Jincho, Y; Sotomaru, Y; et al. Identification of genes aberrantly expressed in mouse embryonic stem cell-cloned blastocysts. BIOLOGY OF REPRODUCTION 78:568-576(2008).

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