cell lysates, tissues lysates
Human, Mouse, Rat
Human/Mouse/Rat MAP2K1 (pS217/221)/Pan MAP2K ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of human, mouse, rat phospho MAP2K1 (S217/221) and Pan MAP2K.
Contents of Kit
1. Mek1 Microplate
2. Wash Buffer Concentrate (20X)
3. Assay Diluent
4. Detection Antibody Mek1 (Ser217/221)
5. Detection Antibody Mek1
6. HRP-conjugated Anti-rabbit IgG
7. TMB One-Step Substrate Reagent
8. Stop Solution
9. Cell Lysate Buffer
10. Positive Control
Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4°C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20°C. Item D-1 store at 2-8°C for up to one month (store at -20°C for up to 6 months, avoid repeated freeze-thaw cycles). Reconstituted Positive Control (Item K) should be stored at -70°C.